Variaties in geneesmiddelgevoeligheid tussen tweedimensionale en driedimensionale traditiemethoden bij triple-negatieve borstkanker bij de meeste kankercellen
Driedimensionale (3D) traditie toont de complexiteit van de tumorbiologie in tegenstelling tot tweedimensionale (2D) traditie. Aldus heeft 3D-traditie aandacht gekregen in celbiologisch onderzoek samen met onderzoeken naar geneesmiddelgevoeligheid.
Hierin hebben we variaties in antikankermedicijngevoeligheden tussen 2D- en 3D-traditiemethoden in triple-negatieve borstkanker (TNBC) celstammen onderzocht. 13 TNBC-celstammen werden drie dagen eerder in stand gehouden in 2D- en 3D-culturen dan de publiciteit voor geneesmiddelen. Celmorfologie binnen de 3D-traditie werd onderzocht met fasecontrastmicroscopie.
Gevoeligheden voor epirubicine (EPI), cisplatine (CDDP) en docetaxel (DTX) zijn onderzocht door middel van cellevensvatbaarheidstest in elke culturen en in tegenstelling daarmee. De IC50’s van alle drie de medicatie zijn aanzienlijk groter geweest binnen de 3D-traditie dan binnen de 2D-traditie in de meeste celstammen.
Deze zijn gecorreleerd tussen de 2D- en 3D-culturen in EPI (R = 0,555) en CDDP (R = 0,955), maar niet in DTX (R = 0,221). Sferische sferoïde-vormende cellen zijn extra bestendig tegen makelaars dan druivensoorten. Concluderend, de 3D-traditie was extra bewijs tegen alle drie de medicatie dan de 2D-traditie in de meeste TNBC-celstammen. Gevoeligheid voor CDDP was extreem gecorreleerd tussen de 2D- en 3D-culturen, maar niet voor DTX. 2D-traditie zou ook acceptabel kunnen zijn voor gevoeligheid, kijk eens naar voor DNA-beschadigende makelaars.
skinsandbones
Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & CleriGelTM; w/
PT017G-5L
EWC Diagnostics
1 unit
EUR 9.73
Description: Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & CleriGelTM; w/ CaCl2, Vitamins, Sucrose & CleriGelTM; w/ CaCl2, Vitamins, Sucrose & CleriGelTM; w/ CaCl2, Vitamins, Sucrose & CleriGelTM
Gamborg B5 Medium w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitam
PT016-100X1L
EWC Diagnostics
1 unit
EUR 85.44
Description: Gamborg B5 Medium w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose
Gamborg B5 Medium w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitam
PT016-10X1L
EWC Diagnostics
1 unit
EUR 10.65
Description: Gamborg B5 Medium w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose
Gamborg B5 Medium w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitam
PT016-25L
EWC Diagnostics
1 unit
EUR 21.81
Description: Gamborg B5 Medium w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose
Gamborg B5 Medium w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitam
PT016-5L
EWC Diagnostics
1 unit
EUR 6.39
Description: Gamborg B5 Medium w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose; w/ CaCl2, Vitamins & Sucrose
Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2,
PT017-10X1L
EWC Diagnostics
1 unit
EUR 17.94
Description: Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2, Vitamins, Sucrose & Agar
Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2,
PT017-25L
EWC Diagnostics
1 unit
EUR 28.74
Description: Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2, Vitamins, Sucrose & Agar
Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2,
PT017-5L
EWC Diagnostics
1 unit
EUR 7.8
Description: Gamborg B5 Medium w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2, Vitamins, Sucrose & Agar; w/ CaCl2, Vitamins, Sucrose & Agar
Murashige and Skoog (MS) Medium, w/ Gamborg B5 Vitamins
30630059-2
Bio-WORLD
25 L
EUR 28.45
Murashige and Skoog (MS) Medium, w/ Gamborg B5 Vitamins
30630059-3
Bio-WORLD
50 L
EUR 55.93
Murashige & Skoog Modified Medium;w/ CaCl₂, Gamborg B5 vitamins
PT114-10X1L
EWC Diagnostics
1 unit
EUR 15.85
Description: Murashige & Skoog Modified Medium;w/ CaCl₂, Gamborg B5 vitamins & Sucrose;w/o Agar
Murashige & Skoog Modified Medium;w/ CaCl₂, Gamborg B5 vitamins
PT114-25L
EWC Diagnostics
1 unit
EUR 23.84
Description: Murashige & Skoog Modified Medium;w/ CaCl₂, Gamborg B5 vitamins & Sucrose;w/o Agar
Murashige & Skoog Modified Medium;w/ CaCl₂, Gamborg B5 vitamins
PT114-5L
EWC Diagnostics
1 unit
EUR 6.89
Description: Murashige & Skoog Modified Medium;w/ CaCl₂, Gamborg B5 vitamins & Sucrose;w/o Agar
Murashige & Skoog Modified Medium w/ CaCl2 & Gamborg B5 vitamin
PT025-100X1L
EWC Diagnostics
1 unit
EUR 80.14
Description: Murashige & Skoog Modified Medium w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins
Murashige & Skoog Modified Medium w/ CaCl2 & Gamborg B5 vitamin
PT025-10X1L
EWC Diagnostics
1 unit
EUR 10.05
Description: Murashige & Skoog Modified Medium w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins
Murashige & Skoog Modified Medium w/ CaCl2 & Gamborg B5 vitamin
PT025-25L
EWC Diagnostics
1 unit
EUR 17.85
Description: Murashige & Skoog Modified Medium w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins
Murashige & Skoog Modified Medium w/ CaCl2 & Gamborg B5 vitamin
PT025-5L
EWC Diagnostics
1 unit
EUR 5.95
Description: Murashige & Skoog Modified Medium w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins; w/ CaCl2 & Gamborg B5 vitamins
Gamborg's B-5 Medium; With Vitamins
CP011-010
GenDepot
10X1L
EUR 121.2
Gamborg's B-5 Medium; With Vitamins
CP011-500
GenDepot
50L
EUR 151.2
Gamborg B5 Vitamins Solution (1000X)
PL018-1X50ML
EWC Diagnostics
1 unit
EUR 16.44
Description: Gamborg B5 Vitamins Solution (1000X)
Gamborg B5 Vitamins Solution (1000X)
PL018-5X50ML
EWC Diagnostics
1 unit
EUR 70.53
Description: Gamborg B5 Vitamins Solution (1000X)
Gamborg's B-5 Medium; With Vitamins and Sucrose
CP012-010
GenDepot
10X1L
EUR 124.8
Gamborg's B-5 Medium; With Vitamins and Sucrose
CP012-500
GenDepot
50L
EUR 151.2
Gamborg B5 Medium
30630028-2
Bio-WORLD
25 L
EUR 27.68
Gamborg B5 Medium
30630028-3
Bio-WORLD
50 L
EUR 46.78
NLN Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins;
PT094-25L
EWC Diagnostics
1 unit
EUR 21.74
Description: NLN Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
NLN Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins;
PT094-5L
EWC Diagnostics
1 unit
EUR 5.78
Description: NLN Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Litvay Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamin
PT042-10X1L
EWC Diagnostics
1 unit
EUR 11.59
Description: Litvay Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Litvay Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamin
PT042-25L
EWC Diagnostics
1 unit
EUR 18.5
Description: Litvay Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Litvay Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamin
PT042-5L
EWC Diagnostics
1 unit
EUR 5.04
Description: Litvay Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Nitsch Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamin
PT143-25L
EWC Diagnostics
1 unit
EUR 20.46
Description: Nitsch Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Nitsch Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamin
PT143-5L
EWC Diagnostics
1 unit
EUR 5.37
Description: Nitsch Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
DKW / Juglans Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/
PT032-10X1L
EWC Diagnostics
1 unit
EUR 11.59
Description: DKW / Juglans Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
DKW / Juglans Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/
PT032-25L
EWC Diagnostics
1 unit
EUR 15.09
Description: DKW / Juglans Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
DKW / Juglans Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/
PT032-5L
EWC Diagnostics
1 unit
EUR 5.04
Description: DKW / Juglans Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Eriksson (ER) Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/
PT033-10X1L
EWC Diagnostics
1 unit
EUR 10.62
Description: Eriksson (ER) Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Eriksson (ER) Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/
PT033-25L
EWC Diagnostics
1 unit
EUR 13.85
Description: Eriksson (ER) Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Eriksson (ER) Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/
PT033-5L
EWC Diagnostics
1 unit
EUR 4.61
Description: Eriksson (ER) Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Murashige & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins;
PT018-100X1L
EWC Diagnostics
1 unit
EUR 72.01
Description: Murashige & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Murashige & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins;
PT018-10X1L
EWC Diagnostics
1 unit
EUR 12.35
Description: Murashige & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Murashige & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins;
PT018-25L
EWC Diagnostics
1 unit
EUR 18.27
Description: Murashige & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Murashige & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins;
PT018-5L
EWC Diagnostics
1 unit
EUR 5.37
Description: Murashige & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Linsmaier & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins;
PT040-10X1L
EWC Diagnostics
1 unit
EUR 11.48
Description: Linsmaier & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Linsmaier & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins;
PT040-25L
EWC Diagnostics
1 unit
EUR 15.28
Description: Linsmaier & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Linsmaier & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins;
PT040-5L
EWC Diagnostics
1 unit
EUR 4.99
Description: Linsmaier & Skoog Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Quoirin & Lepoivre Medium w/ Vitamins; w/ Vitamins; w/ Vitamins
PT056-10X1L
EWC Diagnostics
1 unit
EUR 11.59
Description: Quoirin & Lepoivre Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Quoirin & Lepoivre Medium w/ Vitamins; w/ Vitamins; w/ Vitamins
PT056-25L
EWC Diagnostics
1 unit
EUR 18.53
Description: Quoirin & Lepoivre Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Quoirin & Lepoivre Medium w/ Vitamins; w/ Vitamins; w/ Vitamins
PT056-5L
EWC Diagnostics
1 unit
EUR 5.04
Description: Quoirin & Lepoivre Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Rugini Olive Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ V
PT057-10X1L
EWC Diagnostics
1 unit
EUR 11.59
Description: Rugini Olive Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Rugini Olive Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ V
PT057-25L
EWC Diagnostics
1 unit
EUR 18.5
Description: Rugini Olive Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Rugini Olive Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ V
PT057-5L
EWC Diagnostics
1 unit
EUR 5.04
Description: Rugini Olive Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Westvaco WV5 Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ V
PT086-10X1L
EWC Diagnostics
1 unit
EUR 11.59
Description: Westvaco WV5 Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Westvaco WV5 Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ V
PT086-25L
EWC Diagnostics
1 unit
EUR 18.41
Description: Westvaco WV5 Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Westvaco WV5 Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ V
PT086-5L
EWC Diagnostics
1 unit
EUR 5.04
Description: Westvaco WV5 Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Mitra Orchid Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ V
PT139-25L
EWC Diagnostics
1 unit
EUR 23.26
Description: Mitra Orchid Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Mitra Orchid Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ V
PT139-5L
EWC Diagnostics
1 unit
EUR 6.1
Description: Mitra Orchid Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
De Greef & Jacobs Medium w/ Vitamins; w/ Vitamins; w/ Vitamins;
PT031-10X1L
EWC Diagnostics
1 unit
EUR 11.59
Description: De Greef & Jacobs Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
De Greef & Jacobs Medium w/ Vitamins; w/ Vitamins; w/ Vitamins;
PT031-25L
EWC Diagnostics
1 unit
EUR 15.09
Description: De Greef & Jacobs Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
De Greef & Jacobs Medium w/ Vitamins; w/ Vitamins; w/ Vitamins;
PT031-5L
EWC Diagnostics
1 unit
EUR 5.04
Description: De Greef & Jacobs Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Gamborg B5 Vitamin Mix
30630027-1
Bio-WORLD
100 mL
EUR 41.8
NB Medium;w/ CHU macroelements, Gamborg microelements, Gamborg
PT107-10X1L
EWC Diagnostics
1 unit
EUR 16.05
Description: NB Medium;w/ CHU macroelements, Gamborg microelements, Gamborg B5 vitamins &Sucrose;w/o Agar
NB Medium;w/ CHU macroelements, Gamborg microelements, Gamborg
PT107-25L
EWC Diagnostics
1 unit
EUR 23.93
Description: NB Medium;w/ CHU macroelements, Gamborg microelements, Gamborg B5 vitamins &Sucrose;w/o Agar
NB Medium;w/ CHU macroelements, Gamborg microelements, Gamborg
PT107-5L
EWC Diagnostics
1 unit
EUR 6.98
Description: NB Medium;w/ CHU macroelements, Gamborg microelements, Gamborg B5 vitamins &Sucrose;w/o Agar
Nitsch Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/
PT012-10X1L
EWC Diagnostics
1 unit
EUR 13.67
Description: Nitsch Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose
Nitsch Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/
PT012-25L
EWC Diagnostics
1 unit
EUR 20.46
Description: Nitsch Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose
Nitsch Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/
PT012-5L
EWC Diagnostics
1 unit
EUR 5.95
Description: Nitsch Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose
Street Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/
PT013-10X1L
EWC Diagnostics
1 unit
EUR 13.32
Description: Street Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose
Street Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/
PT013-25L
EWC Diagnostics
1 unit
EUR 19.97
Description: Street Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose
Street Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/
PT013-5L
EWC Diagnostics
1 unit
EUR 5.78
Description: Street Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose
NLN Medium, w/ Vitamins
30630103-2
Bio-WORLD
25 L
EUR 27.12
NLN Medium, w/ Vitamins
30630103-3
Bio-WORLD
50 L
EUR 55.93
Gresshoff & Doy (DBM2) Medium w/ Vitamins; w/ Vitamins; w/ Vita
PT035-10X1L
EWC Diagnostics
1 unit
EUR 11.59
Description: Gresshoff & Doy (DBM2) Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Gresshoff & Doy (DBM2) Medium w/ Vitamins; w/ Vitamins; w/ Vita
PT035-25L
EWC Diagnostics
1 unit
EUR 14.96
Description: Gresshoff & Doy (DBM2) Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Gresshoff & Doy (DBM2) Medium w/ Vitamins; w/ Vitamins; w/ Vita
PT035-5L
EWC Diagnostics
1 unit
EUR 5.04
Description: Gresshoff & Doy (DBM2) Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Litvay Medium, w/ Vitamins
30630049-3
Bio-WORLD
25 L
EUR 41.69
Litvay Medium, w/ Vitamins
30630049-4
Bio-WORLD
50 L
EUR 55.97
White Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ V
PT014-10X1L
EWC Diagnostics
1 unit
EUR 13.67
Description: White Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose
White Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ V
PT014-25L
EWC Diagnostics
1 unit
EUR 20.46
Description: White Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose
White Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ V
PT014-5L
EWC Diagnostics
1 unit
EUR 5.95
Description: White Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose
Chée & Pool (C2D) Vitis Medium w/ Vitamins; w/ Vitamins; w/ Vit
PT027-10X1L
EWC Diagnostics
1 unit
EUR 10.87
Description: Chée & Pool (C2D) Vitis Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Chée & Pool (C2D) Vitis Medium w/ Vitamins; w/ Vitamins; w/ Vit
PT027-25L
EWC Diagnostics
1 unit
EUR 15.16
Description: Chée & Pool (C2D) Vitis Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Chée & Pool (C2D) Vitis Medium w/ Vitamins; w/ Vitamins; w/ Vit
PT027-5L
EWC Diagnostics
1 unit
EUR 4.73
Description: Chée & Pool (C2D) Vitis Medium w/ Vitamins; w/ Vitamins; w/ Vitamins; w/ Vitamins
Murashige & Skoog Medium w/ Vitamins & Sucrose; w/ Vitamins & S
PT010-100X1L
EWC Diagnostics
1 unit
EUR 85.77
Description: Murashige & Skoog Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose
Murashige & Skoog Medium w/ Vitamins & Sucrose; w/ Vitamins & S
PT010-10X1L
EWC Diagnostics
1 unit
EUR 10.52
Description: Murashige & Skoog Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose
Murashige & Skoog Medium w/ Vitamins & Sucrose; w/ Vitamins & S
PT010-25L
EWC Diagnostics
1 unit
EUR 21.81
Description: Murashige & Skoog Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose
Murashige & Skoog Medium w/ Vitamins & Sucrose; w/ Vitamins & S
PT010-5L
EWC Diagnostics
1 unit
EUR 6.39
Description: Murashige & Skoog Medium w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose; w/ Vitamins & Sucrose
Woody Plant Medium w/ CaCl2 & Vitamins; w/ CaCl2 & Vitamins; w/
PT026-10X1L
EWC Diagnostics
1 unit
EUR 24.44
Description: Woody Plant Medium w/ CaCl2 & Vitamins; w/ CaCl2 & Vitamins; w/ CaCl2 & Vitamins; w/ CaCl2 & Vitamins
Woody Plant Medium w/ CaCl2 & Vitamins; w/ CaCl2 & Vitamins; w/
PT026-25L
EWC Diagnostics
1 unit
EUR 36.86
Description: Woody Plant Medium w/ CaCl2 & Vitamins; w/ CaCl2 & Vitamins; w/ CaCl2 & Vitamins; w/ CaCl2 & Vitamins
Woody Plant Medium w/ CaCl2 & Vitamins; w/ CaCl2 & Vitamins; w/
PT026-5L
EWC Diagnostics
1 unit
EUR 10.7
Description: Woody Plant Medium w/ CaCl2 & Vitamins; w/ CaCl2 & Vitamins; w/ CaCl2 & Vitamins; w/ CaCl2 & Vitamins
CHU (N6) Medium;w/ Vitamins
PT030-100X1L
EWC Diagnostics
1 unit
EUR 71.61
Description: CHU (N6) Medium;w/ Vitamins
CHU (N6) Medium;w/ Vitamins
PT030-10X1L
EWC Diagnostics
1 unit
EUR 11.79
Description: CHU (N6) Medium;w/ Vitamins
CHU (N6) Medium;w/ Vitamins
PT030-25L
EWC Diagnostics
1 unit
EUR 15.37
Description: CHU (N6) Medium;w/ Vitamins
CHU (N6) Medium;w/ Vitamins
PT030-5L
EWC Diagnostics
1 unit
EUR 5.13
Description: CHU (N6) Medium;w/ Vitamins
Chu (N6) Medium, w/ Vitamins
30630008-2
Bio-WORLD
25 L
EUR 32.39
Chu (N6) Medium, w/ Vitamins
30630008-3
Bio-WORLD
50 L
EUR 54.68
DKW/Juglans Medium, w/ Vitamins
30630020-3
Bio-WORLD
25 L
EUR 41.69
DKW/Juglans Medium, w/ Vitamins
30630020-4
Bio-WORLD
50 L
EUR 77.96
Eriksson (ER) Medium, w/ Vitamins
30630024-3
Bio-WORLD
25 L
EUR 41.69
Eriksson (ER) Medium, w/ Vitamins
30630024-4
Bio-WORLD
50 L
EUR 67.71
Gamborg B5 Vitamin Concentrate (Syringe Vials)
30630026-1
Bio-WORLD
100 mL
EUR 55.96
Murashige & Skoog/Gamborg (MG) Medium
30630206-2
Bio-WORLD
25 L
EUR 36.31
Murashige & Skoog/Gamborg (MG) Medium
30630206-3
Bio-WORLD
50 L
EUR 61.8
Anderson's Rhododendron Medium, w/ Vitamins
30630000-2
Bio-WORLD
25 L
EUR 26.43
Anderson's Rhododendron Medium, w/ Vitamins
30630000-3
Bio-WORLD
50 L
EUR 36.26
Quoirin and Lepoivre Medium, w/ Vitamins
30630108-3
Bio-WORLD
25 L
EUR 49.77
Quoirin and Lepoivre Medium, w/ Vitamins
30630108-4
Bio-WORLD
50 L
EUR 93.8
Nitsch Medium;w/ Vitamins;w/o Sucrose & Agar
PT143-10X1L
EWC Diagnostics
1 unit
EUR 12.35
Description: Nitsch Medium;w/ Vitamins;w/o Sucrose & Agar
Schenk and Hildebrandt Medium, w/ Vitamins
30630135-3
Bio-WORLD
25 L
EUR 33.59
Schenk and Hildebrandt Medium, w/ Vitamins
30630135-4
Bio-WORLD
50 L
EUR 57.88
CHU (N₆) Medium;w/ Vitamins & Sucrose;w/o Agar
PT113-10X1L
EWC Diagnostics
1 unit
EUR 13.82
Description: CHU (N₆) Medium;w/ Vitamins & Sucrose;w/o Agar
CHU (N₆) Medium;w/ Vitamins & Sucrose;w/o Agar
PT113-25L
EWC Diagnostics
1 unit
EUR 18.01
Description: CHU (N₆) Medium;w/ Vitamins & Sucrose;w/o Agar
CHU (N₆) Medium;w/ Vitamins & Sucrose;w/o Agar
PT113-5L
EWC Diagnostics
1 unit
EUR 6.01
Description: CHU (N₆) Medium;w/ Vitamins & Sucrose;w/o Agar
De Greef and Jacobs Medium, w/ Vitamins
30630016-3
Bio-WORLD
25 L
EUR 41.69
De Greef and Jacobs Medium, w/ Vitamins
30630016-4
Bio-WORLD
50 L
EUR 62.21
CLC/Ipomoea Basal Medium CP, w/ Vitamins
30630011-3
Bio-WORLD
25 L
EUR 70.9
CLC/Ipomoea Basal Medium CP, w/ Vitamins
30630011-4
Bio-WORLD
50 L
EUR 89.55
CLC/Ipomoea Basal Medium EP, w/ Vitamins
30630012-3
Bio-WORLD
25 L
EUR 41.06
CLC/Ipomoea Basal Medium EP, w/ Vitamins
30630012-4
Bio-WORLD
50 L
EUR 55.97
Hoagland & Knop Medium;w/ Agar;w/o Vitamins
PT126-10X1L
EWC Diagnostics
1 unit
EUR 13.67
Description: Hoagland & Knop Medium;w/ Agar;w/o Vitamins
Hoagland & Knop Medium;w/ Agar;w/o Vitamins
PT126-25L
EWC Diagnostics
1 unit
EUR 20.37
Description: Hoagland & Knop Medium;w/ Agar;w/o Vitamins
Hoagland & Knop Medium;w/ Agar;w/o Vitamins
PT126-5L
EWC Diagnostics
1 unit
EUR 5.95
Description: Hoagland & Knop Medium;w/ Agar;w/o Vitamins
Carnation Rooting Medium w/ Vitamins, Sucrose & Agar w/ Vitamin
PT123-10X1L
EWC Diagnostics
1 unit
EUR 20.17
Description: Carnation Rooting Medium w/ Vitamins, Sucrose & Agar w/ Vitamins, Sucrose & Agar w/ Vitamins, Sucrose & Agar w/ Vitamins, Sucrose & Agar
Carnation Rooting Medium w/ Vitamins, Sucrose & Agar w/ Vitamin
PT123-25L
EWC Diagnostics
1 unit
EUR 30.05
Description: Carnation Rooting Medium w/ Vitamins, Sucrose & Agar w/ Vitamins, Sucrose & Agar w/ Vitamins, Sucrose & Agar w/ Vitamins, Sucrose & Agar
Carnation Rooting Medium w/ Vitamins, Sucrose & Agar w/ Vitamin
PT123-5L
EWC Diagnostics
1 unit
EUR 8.77
Description: Carnation Rooting Medium w/ Vitamins, Sucrose & Agar w/ Vitamins, Sucrose & Agar w/ Vitamins, Sucrose & Agar w/ Vitamins, Sucrose & Agar
Gresshoff and Doy (DBM2) Medium, w/ Vitamins
30630035-3
Bio-WORLD
25 L
EUR 41.69
Gresshoff and Doy (DBM2) Medium, w/ Vitamins
30630035-4
Bio-WORLD
50 L
EUR 64.34
Litvay Medium;w/ Sucrose & Agar;w/o Vitamins
PT140-10X1L
EWC Diagnostics
1 unit
EUR 15.75
Description: Litvay Medium;w/ Sucrose & Agar;w/o Vitamins
Murashige & Skoog Medium w/ Vitamins, Sucrose & Agar; w/ Vitami
PT011-100X1L
EWC Diagnostics
1 unit
EUR 87
Description: Murashige & Skoog Medium w/ Vitamins, Sucrose & Agar; w/ Vitamins, Sucrose & Agar; w/ Vitamins, Sucrose & Agar; w/ Vitamins, Sucrose & Agar
Murashige & Skoog Medium w/ Vitamins, Sucrose & Agar; w/ Vitami
PT011-10X1L
EWC Diagnostics
1 unit
EUR 10.79
Description: Murashige & Skoog Medium w/ Vitamins, Sucrose & Agar; w/ Vitamins, Sucrose & Agar; w/ Vitamins, Sucrose & Agar; w/ Vitamins, Sucrose & Agar
Murashige & Skoog Medium w/ Vitamins, Sucrose & Agar; w/ Vitami
PT011-25L
EWC Diagnostics
1 unit
EUR 22.29
Description: Murashige & Skoog Medium w/ Vitamins, Sucrose & Agar; w/ Vitamins, Sucrose & Agar; w/ Vitamins, Sucrose & Agar; w/ Vitamins, Sucrose & Agar
Murashige & Skoog Medium w/ Vitamins, Sucrose & Agar; w/ Vitami
PT011-5L
EWC Diagnostics
1 unit
EUR 6.5
Description: Murashige & Skoog Medium w/ Vitamins, Sucrose & Agar; w/ Vitamins, Sucrose & Agar; w/ Vitamins, Sucrose & Agar; w/ Vitamins, Sucrose & Agar
DKW / Juglans Medium;w/ Vitamins & Sucrose;w/o Agar
PT147-10X1L
EWC Diagnostics
1 unit
EUR 12.35
Description: DKW / Juglans Medium;w/ Vitamins & Sucrose;w/o Agar
DKW / Juglans Medium;w/ Vitamins & Sucrose;w/o Agar
PT147-25L
EWC Diagnostics
1 unit
EUR 15.75
Description: DKW / Juglans Medium;w/ Vitamins & Sucrose;w/o Agar
DKW / Juglans Medium;w/ Vitamins & Sucrose;w/o Agar
PT147-5L
EWC Diagnostics
1 unit
EUR 5.37
Description: DKW / Juglans Medium;w/ Vitamins & Sucrose;w/o Agar
Eriksson (ER) Medium;w/ Vitamins & Sucrose;w/o Agar
PT148-10X1L
EWC Diagnostics
1 unit
EUR 12.45
Description: Eriksson (ER) Medium;w/ Vitamins & Sucrose;w/o Agar
Eriksson (ER) Medium;w/ Vitamins & Sucrose;w/o Agar
PT148-25L
EWC Diagnostics
1 unit
EUR 14.67
Description: Eriksson (ER) Medium;w/ Vitamins & Sucrose;w/o Agar
Eriksson (ER) Medium;w/ Vitamins & Sucrose;w/o Agar
PT148-5L
EWC Diagnostics
1 unit
EUR 5.42
Description: Eriksson (ER) Medium;w/ Vitamins & Sucrose;w/o Agar
Westvaco WV3 Medium,w/ Vitamins;w/o Sucrose & Agar
PT085-10X1L
EWC Diagnostics
1 unit
EUR 11.59
Description: Westvaco WV3 Medium,w/ Vitamins;w/o Sucrose & Agar
Westvaco WV3 Medium,w/ Vitamins;w/o Sucrose & Agar
PT085-25L
EWC Diagnostics
1 unit
EUR 18.41
Description: Westvaco WV3 Medium,w/ Vitamins;w/o Sucrose & Agar
Westvaco WV3 Medium,w/ Vitamins;w/o Sucrose & Agar
PT085-5L
EWC Diagnostics
1 unit
EUR 5.04
Description: Westvaco WV3 Medium,w/ Vitamins;w/o Sucrose & Agar
Mitra Orchid Medium;w/ Vitamins & Sucrose;w/o Agar
PT106-10X1L
EWC Diagnostics
1 unit
EUR 17.94
Description: Mitra Orchid Medium;w/ Vitamins & Sucrose;w/o Agar
Mitra Orchid Medium;w/ Vitamins & Sucrose;w/o Agar
PT106-25L
EWC Diagnostics
1 unit
EUR 28.82
Description: Mitra Orchid Medium;w/ Vitamins & Sucrose;w/o Agar
Mitra Orchid Medium;w/ Vitamins & Sucrose;w/o Agar
PT106-5L
EWC Diagnostics
1 unit
EUR 7.8
Description: Mitra Orchid Medium;w/ Vitamins & Sucrose;w/o Agar
Ontwikkeling van een extracellulaire matrix-mannequin voor 3D-geest om het samenspel tussen microglia en T-cellen in co-traditie te beoordelen
Neurodegeneratieve problemen worden gekenmerkt door de activering van in de hersenen aanwezige microgliacellen en door de infiltratie van perifere T-cellen. Desalniettemin is hun interactie bij ziekte niet opgehelderd, maar. Het is moeilijk om gecompliceerde mobiele dynamiek bij levende dieren te onderzoeken, en eenvoudige tweedimensionale (2D) celtraditie-modes lijken niet op de delicate 3D-constructie van hersenweefsel. Vervolgens hebben we een biomimetisch 3D in vitro traditiesysteem ontwikkeld voor het samen kweken van microglia- en T-cellen.
Omdat activering en / of migratie van immuuncellen in de geest mogelijk wordt beïnvloed door delen van de extracellulaire matrix, zijn geschetste 3D-fibrillaire op collageen I gebaseerde matrices geconstrueerd en gemodificeerd met hyaluronan en / of chondroïtinesulfaat, die lijken op de punten van de geest. extracellulaire matrix.
Muizenmicroglia en milt-afgeleide T-cellen zijn alleen of in co-cultuur op de geconstrueerde matrices gekweekt.
Microglia vertoonde een in vivo-achtige morfologie en T-cellen bevestigden verbeterde overleving wanneer ze samen met microglia werden gekweekt of tot een klein diploma in de aanwezigheid van glia-geconditioneerd medium.De open en poreuze fibrillaire constructie van de matrix maakte celinvasie en directe cel-cel interactie mogelijk, met een sterkere invasie van T-cellen. Elke celsortering bevestigde geen afhankelijkheid van de matrixmodificaties. Microglia kan op de matrices worden geactiveerd door lipopolysaccharide, wat leidt tot secretie van interleukine-6 en tumornecrosefactor-α. De bevindingen hierin wijzen erop dat biomimetische 3D-matrices co-cultivatie en activering van belangrijke microglia- en T-cellen mogelijk maken en nuttige instrumenten leveren om hun interactie in vitro te beoordelen.
Een tweedimensionale multiwell celcultuurmethode voor de productie van CYP3A4 tot expressie brengende hepatocyte-achtige cellen uit HepaRG-cellen
Cytochroom P450-enzymen (CYP) werken bij het metabolisme van geneesmiddelen in de lever. Om een flink aantal kandidaat-geneesmiddelen te bepalen, is een high-content screening (HCS) -systeem met hepatocyt-achtige cellen (HLC’s) nodig die volwassen menselijke hepatocyten kunnen veranderen. Humaan hepatocellulair carcinoom HepaRG is de enige cellijn die HLC’s kan aanbieden met overmatige CYP3A4-expressie, vergelijkbaar met die in volwassen hepatocyten na celdifferentiatie.
Het doel van dit onderzoek was om een geweldig multiwell-traditiesysteem te ontwerpen voor HLC’s met behulp van transgene HepaRG-cellen die het EGFP tot expressie brengen dat codeert voor een verbeterd onervaren fluorescerend eiwit onder CYP3A4-transcriptieregulatie. HLC’s zijn gerijpt op 5 verschillende soorten 96-wells zwarte platen.
Het kweken van HLC’s op optische CVG-platen met glazen bodem bevorderde de celrijping en verhoogde metabolische inspanning aanzienlijk door tweevoudig onder tweedimensionale (2D) traditiesituaties, en deze opties zijn verbeterd door een coating van 2% collageen.
Drie platen voor driedimensionale (3D) celculturen met een gasuitwisselbare doek of dimethylpolysiloxaanmembraan aan de achterkant vormden een aantal bolvormige kolonies, terwijl ze ineff
Proces van PCR
De PCR bestaat uit een proces dat bestaat uit een reeks van temperatuurwisselingen die 25-50 keer wordt herhaald. Er zijn drie fasen van de cyclus, de eerste fase maakt de scheiding van de dubbele nucleïnezuurketen mogelijk bij ongeveer 95 * C, en de tweede fase maakt de binding van de primers mogelijk met behulp van een DNA-sjabloon bij een temperatuur van ongeveer 50 * -60 * C en de derde fase vergemakkelijkte de polymerisatie die wordt uitgevoerd door het DNA-polymerase bij een temperatuur tussen 68-72 ° C.
Er is de kleine omvang van de fragmenten, dus de laatste stap is het type PCR dat hun aantal kan verhogen tijdens de uitlijningsfase en de denaturatiestap en de vier stappen worden gemeten tijdens korte temperatuurfasen die slechts een paar seconden duren met de temperatuur rond 80 * C om de aanwezigheid van primerdimeren te verminderen.
Niet-specifieke detectie – real-time PCR met dubbelstrengs DNA
Een DNA-bindende kleurstof is een kleurstof die bij PCR aan alle dubbelstrengs DNA bindt, en de opbrengst van de kleurstof verhoogt ook het fluorescentiekwantum. de toename van de DNA-productie tijdens de PCR-tijd leidt tot een toename van de intensiteit van de fluorescentie die bij elke cyclus wordt gemeten. Er zijn echter enkele kleurstoffen zoals SYBR Green die alle dsDNA PCR-producten bindt die ook niet-specifieke PCR-producten bevatten.
De reactie van real-time PCR is al zoals gewoonlijk voorbereid, met toevoeging van fluorescerend dsDNA. Real-time PCR-instrument meet de intensiteit van fluorescentie met een detector, de kleurstof fluoresceert alleen wanneer deze is gebonden aan het dsDNA. Deze methode heeft het perfecte voordeel dat er slechts een paar primers nodig zijn die de amplificatie uitvoeren, wat ook de kosten laag houdt en er zijn meerdere doelen die in een buis kunnen worden bewaakt door verschillende soorten kleurstoffen te gebruiken.
