Abstrakt
Eines der wichtigsten Probleme in Bezug auf die Leistung von PCR-Verbrauchsmaterialien (Platten, Streifen und entsprechende Versiegelungsoptionen) ist ihre Kompatibilität mit mehreren auf dem Markt erhältlichen Cycler-Systemen. In dieser Application Note wurden Eppendorf PCR/qPCR Consumables auf Kompatibilität mit den wichtigsten Cycler-Marken durch einen Physical-Fit-Test mit den Cycler-Blöcken sowie durch exemplarische PCR- und qPCR-Assays für einige Instrumente getestet.
Einführung
PCR- und qPCR-Verbrauchsmaterialien unterscheiden sich in zahlreichen Parametern. Darunter spielen Materialeigenschaften und -qualität, Brunnen-/Schürzenabmessungen, Brunnendicke und Oberflächenbeschaffenheit die wichtigste Rolle. Diese Unterschiede führen zusammen mit verschiedenen Versiegelungsoptionen zu oft erheblichen Abweichungen beim Wechsel von einer Verbrauchsartikelmarke zu einer anderen und können die Gesamtleistung des PCR/qPCR-Assays beeinflussen. Daher ist es von großer Bedeutung festzustellen, ob ein bestimmtes Verbrauchsmaterial mit einer bestimmten PCR/qPCR-Plattform kompatibel ist. Hier präsentieren wir Ihnen einen Überblick über die Eppendorf twin.tec PCR Plates, Eppendorf PCR Tube Strips sowie Eppendorf Cap Strips und ihre Kompatibilität mit den wichtigsten derzeit auf dem Markt befindlichen Cycler-Marken.
Materialen und Methoden
Kompatibilität mit Standard-PCR- und qPCR-Geräten In den meisten Fällen wurde die Kompatibilität der Eppendorf PCR Consumables durch ihre Passung in den entsprechenden Cycler-Block in Kombination mit dem Verschluss des Cycler-Deckels bestimmt. Einige Systeme wurden auch auf ihre Leistungsfähigkeit in einem PCR- oder qPCR-Assay getestet.
Natürlich sind diese Experimente nicht repräsentativ. Daher wird es für jeden Anwender immer notwendig sein, die Verbrauchsmaterialien in seiner eigenen Anwendung zu testen. PCR-Assay: Amplifikation von gDNA (Promega) in einer PCR. Nach dem PCR-Assay wurden die Proben mittels Agarose-Gelelektrophorese (E-Gel, Invitrogen) aufgetrennt und dokumentiert.
Kompatibilität und Testleistung wurden anhand des Vorhandenseins und der Qualität von PCR-Produkten auf dem Gel bewertet (Daten nicht gezeigt).
for BioRad i-Serie, Stratagene Mx, ABI 3000 (excl. adapter) |
|||
| A28242L | GeneOn | 5x25 pcs | 249 EUR |
ABI 3000, 7000; BioRad i-Serie, Stratagene Mx, Eppendorf Mastercycler |
|||
| A28239L | GeneOn | 5x25 pcs | 249 EUR |
Stratagene Mx (incl. Perfect Fit Frames from Stratagene), Biorad CFX 96 |
|||
| A28240L | GeneOn | 5x25 pcs | 249 EUR |
Sequencer; ABI 3000, 7000 7900HT; Stratagene Mx4000 and other |
|||
| A20232L | GeneOn | 5x25 pcs | 269 EUR |
1ml PrecisionPLUS qPCR Master Mix for Stratagene hardware |
|||
| Z-PPLUS-MX-1ML | Novacyt Group | 1ml | 81 EUR |
1ml Precision FAST qPCR Master Mix for Stratagene hardware |
|||
| Z-PFAST-MX-1ML | Novacyt Group | 1ml | 90 EUR |
2ml Precision FAST qPCR Master Mix for Stratagene hardware |
|||
| Z-PFAST-MX-2ML | Novacyt Group | 2ml | 161 EUR |
BioRad CFX96, Megabase 500/1000 |
|||
| A28241L | GeneOn | 5x25 pcs | 249 EUR |
ALF14-25 Adapter DATA I/O for PSV7000 series AI AIDC |
|||
| 235486 | BRADY NV | Pack of 1 Piece(s) | 1837.5 EUR |
Universal Stratus Adapter - EACH |
|||
| MIC6036 | Scientific Laboratory Supplies | EACH | 666.9 EUR |
ALF14-25 Universal Advantis Series Adapter AI AIDC |
|||
| 199970 | BRADY NV | Pack of 1 Piece(s) | 2730 EUR |
Universal adapter for 5mL tube (58 series) set of 8 - EACH |
|||
| E5820732008 | Scientific Laboratory Supplies | EACH | 31.05 EUR |
1ml PrecisionPLUS qPCR Master Mix for BioRad iCycler |
|||
| Z-PPLUS-iC-1ML | Novacyt Group | 1ml | 81 EUR |
20ml PrecisionPLUS qPCR Master Mix for BioRad iCycler |
|||
| Z-PPLUS-iC-20ML | Novacyt Group | 20ml | 1025 EUR |
ALF14-25 Adapter for ASM Siplace X and SX Series AI AIDC |
|||
| 198684 | BRADY NV | Pack of 1 Piece(s) | 1521.45 EUR |
1ml Precision FAST qPCR Master Mix for BioRad iCycler |
|||
| Z-PFAST-iC-1ML | Novacyt Group | 1ml | 90 EUR |
2ml Precision FAST qPCR Master Mix for BioRad iCycler |
|||
| Z-PFAST-iC-2ML | Novacyt Group | 2ml | 161 EUR |
ALF14-25 Adapter for ASM Siplace X and E-by Series AI AIDC |
|||
| 198524 | BRADY NV | Pack of 1 Piece(s) | 1774.5 EUR |
ALF14-40 Adapter for ASM Siplace X and E-by Series AI AIDC |
|||
| 198685 | BRADY NV | Pack of 1 Piece(s) | 1879.5 EUR |
ALF14-55 Adapter for ASM Siplace X and E-by Series AI AIDC |
|||
| 198686 | BRADY NV | Pack of 1 Piece(s) | 1984.5 EUR |
Adapter for 8x5 mL tubes for rotor S-4-72 (58 series) set of 2 - EACH |
|||
| E5804793005 | Scientific Laboratory Supplies | EACH | 233.55 EUR |
USB2.0 to Serial Adapter |
|||
| AJ846-070-925 | Westburg | each | 74.67 EUR |
Adapter for 1x5 mL tubes for rotor F-34-6-38 (58 series) set of 2 - EACH |
|||
| E5804777000 | Scientific Laboratory Supplies | EACH | 101.25 EUR |
Adapter for 1x5 mL tubesfor rotor FA-45-6-30 (58 series) setof 2 - EACH |
|||
| E5820730005 | Scientific Laboratory Supplies | EACH | 101.25 EUR |
Adapter for 1x5.5-2mL tubes for rotor (58 series) FA-45-20-17 set of 4 - EACH |
|||
| E5820768002 | Scientific Laboratory Supplies | EACH | 41.85 EUR |
Adapter for cryogenic tubes for rotor FA-45-20-17 (58 series) setof 4 - EACH |
|||
| E5820769009 | Scientific Laboratory Supplies | EACH | 41.31 EUR |
Adapter for auto sampler vials for rotor FA-45-20-17 (58 series) set of 4 - EACH |
|||
| E5820770007 | Scientific Laboratory Supplies | EACH | 44.55 EUR |
Adapter for Safe-Lock Tube 5.0 mL for rotor A-4-38 (5702 series) round buckets set of 2 - EACH |
|||
| E5702733008 | Scientific Laboratory Supplies | EACH | 109.35 EUR |
SLC4A1AP (Kanadaptin, Human Lung Cancer Oncogene 3 Protein, HLC-3, Kidney Anion Exchanger Adapter Protein, Solute Carrier Family 4 Anion Exchanger Member 1 Adapter Protein, HLC3) (Biotin) |
|||
| MBS6144439-01mL | MyBiosource | 0.1(mL | 875 EUR |
SLC4A1AP (Kanadaptin, Human Lung Cancer Oncogene 3 Protein, HLC-3, Kidney Anion Exchanger Adapter Protein, Solute Carrier Family 4 Anion Exchanger Member 1 Adapter Protein, HLC3) (Biotin) |
|||
| MBS6144439-5x01mL | MyBiosource | 5x0.1mL | 3800 EUR |
Adapter for Dispenser GL - Adapter, 45/40 mm |
|||
| LHD-A1 | Phoenix instrument | each | 11.66 EUR |
Adapter for Dispenser GL - Adapter, 45/32 mm |
|||
| LHD-A3 | Phoenix instrument | each | 11.66 EUR |
Adapter for Dispenser GL - Adapter, 45/38 mm |
|||
| LHD-A5 | Phoenix instrument | each | 11.66 EUR |
Adapter for Dispenser GL - Adapter, 32/25 mm |
|||
| LHD-A7 | Phoenix instrument | each | 11.66 EUR |
Adapter for Dispenser GL - Adapter, 32/28 mm |
|||
| LHD-A9 | Phoenix instrument | 32/28 mm | 11.66 EUR |
STRADA (LYK5, STE20-related Kinase Adapter Protein alpha, STRAD alpha, STE20-related Adapter Protein, Serologically Defined Breast Cancer Antigen NY-BR-96, STRAD, FLJ90524) (Biotin) |
|||
| MBS6142769-01mL | MyBiosource | 0.1(mL | 875 EUR |
STRADA (LYK5, STE20-related Kinase Adapter Protein alpha, STRAD alpha, STE20-related Adapter Protein, Serologically Defined Breast Cancer Antigen NY-BR-96, STRAD, FLJ90524) (Biotin) |
|||
| MBS6142769-5x01mL | MyBiosource | 5x0.1mL | 3800 EUR |
QUICKFIT(R) STRAIGHT TUBING ADAPTER JOI - PK5 |
|||
| Z322237-5X1EA | Scientific Laboratory Supplies | PK5 | 193.05 EUR |
STRADA (LYK5, STE20-related Kinase Adapter Protein alpha, STRAD alpha, STE20-related Adapter Protein, Serologically Defined Breast Cancer Antigen NY-BR-96, STRAD, FLJ90524) |
|||
| MBS643791-01mg | MyBiosource | 0.1mg | 630 EUR |
STRADA (LYK5, STE20-related Kinase Adapter Protein alpha, STRAD alpha, STE20-related Adapter Protein, Serologically Defined Breast Cancer Antigen NY-BR-96, STRAD, FLJ90524) |
|||
| MBS643791-5x01mg | MyBiosource | 5x0.1mg | 2680 EUR |
SLC4A1AP (Kanadaptin, Human Lung Cancer Oncogene 3 Protein, HLC-3, Kidney Anion Exchanger Adapter Protein, Solute Carrier Family 4 Anion Exchanger Member 1 Adapter Protein, HLC3) (HRP) |
|||
| MBS6155045-01mL | MyBiosource | 0.1(mL | 875 EUR |
SLC4A1AP (Kanadaptin, Human Lung Cancer Oncogene 3 Protein, HLC-3, Kidney Anion Exchanger Adapter Protein, Solute Carrier Family 4 Anion Exchanger Member 1 Adapter Protein, HLC3) (HRP) |
|||
| MBS6155045-5x01mL | MyBiosource | 5x0.1mL | 3800 EUR |
SLC4A1AP (Kanadaptin, Human Lung Cancer Oncogene 3 Protein, HLC-3, Kidney Anion Exchanger Adapter Protein, Solute Carrier Family 4 Anion Exchanger Member 1 Adapter Protein, HLC3) (PE) |
|||
| MBS6160348-01mL | MyBiosource | 0.1(mL | 875 EUR |
SLC4A1AP (Kanadaptin, Human Lung Cancer Oncogene 3 Protein, HLC-3, Kidney Anion Exchanger Adapter Protein, Solute Carrier Family 4 Anion Exchanger Member 1 Adapter Protein, HLC3) (PE) |
|||
| MBS6160348-5x01mL | MyBiosource | 5x0.1mL | 3800 EUR |
SLC4A1AP (Kanadaptin, Human Lung Cancer Oncogene 3 Protein, HLC-3, Kidney Anion Exchanger Adapter Protein, Solute Carrier Family 4 Anion Exchanger Member 1 Adapter Protein, HLC3) APC |
|||
| MBS6139136-01mL | MyBiosource | 0.1(mL | 875 EUR |
SLC4A1AP (Kanadaptin, Human Lung Cancer Oncogene 3 Protein, HLC-3, Kidney Anion Exchanger Adapter Protein, Solute Carrier Family 4 Anion Exchanger Member 1 Adapter Protein, HLC3) APC |
|||
| MBS6139136-5x01mL | MyBiosource | 5x0.1mL | 3800 EUR |
SLC4A1AP (Kanadaptin, Human Lung Cancer Oncogene 3 Protein, HLC-3, Kidney Anion Exchanger Adapter Protein, Solute Carrier Family 4 Anion Exchanger Member 1 Adapter Protein, HLC3) (AP) |
|||
| MBS6133833-01mL | MyBiosource | 0.1(mL | 875 EUR |
SLC4A1AP (Kanadaptin, Human Lung Cancer Oncogene 3 Protein, HLC-3, Kidney Anion Exchanger Adapter Protein, Solute Carrier Family 4 Anion Exchanger Member 1 Adapter Protein, HLC3) (AP) |
|||
| MBS6133833-5x01mL | MyBiosource | 5x0.1mL | 3800 EUR |
SLC4A1AP (Kanadaptin, Human Lung Cancer Oncogene 3 Protein, HLC-3, Kidney Anion Exchanger Adapter Protein, Solute Carrier Family 4 Anion Exchanger Member 1 Adapter Protein, HLC3) (FITC) |
|||
| MBS6149742-01mL | MyBiosource | 0.1(mL | 875 EUR |
SLC4A1AP (Kanadaptin, Human Lung Cancer Oncogene 3 Protein, HLC-3, Kidney Anion Exchanger Adapter Protein, Solute Carrier Family 4 Anion Exchanger Member 1 Adapter Protein, HLC3) (FITC) |
|||
| MBS6149742-5x01mL | MyBiosource | 5x0.1mL | 3800 EUR |
STRADA (LYK5, STE20-related Kinase Adapter Protein alpha, STRAD alpha, STE20-related Adapter Protein, Serologically Defined Breast Cancer Antigen NY-BR-96, STRAD, FLJ90524) (FITC) |
|||
| MBS6148072-01mL | MyBiosource | 0.1(mL | 875 EUR |
STRADA (LYK5, STE20-related Kinase Adapter Protein alpha, STRAD alpha, STE20-related Adapter Protein, Serologically Defined Breast Cancer Antigen NY-BR-96, STRAD, FLJ90524) (FITC) |
|||
| MBS6148072-5x01mL | MyBiosource | 5x0.1mL | 3800 EUR |
STRADA (LYK5, STE20-related Kinase Adapter Protein alpha, STRAD alpha, STE20-related Adapter Protein, Serologically Defined Breast Cancer Antigen NY-BR-96, STRAD, FLJ90524) (PE) |
|||
| MBS6158678-01mL | MyBiosource | 0.1(mL | 875 EUR |
STRADA (LYK5, STE20-related Kinase Adapter Protein alpha, STRAD alpha, STE20-related Adapter Protein, Serologically Defined Breast Cancer Antigen NY-BR-96, STRAD, FLJ90524) (PE) |
|||
| MBS6158678-5x01mL | MyBiosource | 5x0.1mL | 3800 EUR |
STRADA (LYK5, STE20-related Kinase Adapter Protein alpha, STRAD alpha, STE20-related Adapter Protein, Serologically Defined Breast Cancer Antigen NY-BR-96, STRAD, FLJ90524) (HRP) |
|||
| MBS6153375-01mL | MyBiosource | 0.1(mL | 875 EUR |
STRADA (LYK5, STE20-related Kinase Adapter Protein alpha, STRAD alpha, STE20-related Adapter Protein, Serologically Defined Breast Cancer Antigen NY-BR-96, STRAD, FLJ90524) (HRP) |
|||
| MBS6153375-5x01mL | MyBiosource | 5x0.1mL | 3800 EUR |
STRADA (LYK5, STE20-related Kinase Adapter Protein alpha, STRAD alpha, STE20-related Adapter Protein, Serologically Defined Breast Cancer Antigen NY-BR-96, STRAD, FLJ90524) (AP) |
|||
| MBS6132163-01mL | MyBiosource | 0.1(mL | 875 EUR |
STRADA (LYK5, STE20-related Kinase Adapter Protein alpha, STRAD alpha, STE20-related Adapter Protein, Serologically Defined Breast Cancer Antigen NY-BR-96, STRAD, FLJ90524) (AP) |
|||
| MBS6132163-5x01mL | MyBiosource | 5x0.1mL | 3800 EUR |
STRADA (LYK5, STE20-related Kinase Adapter Protein alpha, STRAD alpha, STE20-related Adapter Protein, Serologically Defined Breast Cancer Antigen NY-BR-96, STRAD, FLJ90524) APC |
|||
| MBS6137466-01mL | MyBiosource | 0.1(mL | 875 EUR |
STRADA (LYK5, STE20-related Kinase Adapter Protein alpha, STRAD alpha, STE20-related Adapter Protein, Serologically Defined Breast Cancer Antigen NY-BR-96, STRAD, FLJ90524) APC |
|||
| MBS6137466-5x01mL | MyBiosource | 5x0.1mL | 3800 EUR |
STRADA (STE20-related Kinase Adapter Protein alpha, STRAD alpha, STRAD, STE20-related Adapter Protein, LYK5, PMSE, Serologically Defined Breast Cancer Antigen NY-BR-96, Stlk) |
|||
| MBS6010816-01mL | MyBiosource | 0.1(mL | 675 EUR |
STRADA (STE20-related Kinase Adapter Protein alpha, STRAD alpha, STRAD, STE20-related Adapter Protein, LYK5, PMSE, Serologically Defined Breast Cancer Antigen NY-BR-96, Stlk) |
|||
| MBS6010816-5x01mL | MyBiosource | 5x0.1mL | 2880 EUR |
RTS 500 Adapter |
|||
| BRBR1401901 | Westburg | each | 126.99 EUR |
Adapter for 4 Reagent Strips |
|||
| AJ845-60006-0 | Westburg | each | 358.07 EUR |
SLC4A1AP (Kanadaptin, Human Lung Cancer Oncogene 3 Protein, HLC-3, Kidney Anion Exchanger Adapter Protein, Solute Carrier Family 4 Anion Exchanger Member 1 Adapter Protein, HLC3) (MaxLight 650) |
|||
| MBS6224722-01mL | MyBiosource | 0.1(mL | 875 EUR |
SLC4A1AP (Kanadaptin, Human Lung Cancer Oncogene 3 Protein, HLC-3, Kidney Anion Exchanger Adapter Protein, Solute Carrier Family 4 Anion Exchanger Member 1 Adapter Protein, HLC3) (MaxLight 650) |
|||
| MBS6224722-5x01mL | MyBiosource | 5x0.1mL | 3800 EUR |
SLC4A1AP (Kanadaptin, Human Lung Cancer Oncogene 3 Protein, HLC-3, Kidney Anion Exchanger Adapter Protein, Solute Carrier Family 4 Anion Exchanger Member 1 Adapter Protein, HLC3) (MaxLight 405) |
|||
| MBS6192697-01mL | MyBiosource | 0.1(mL | 875 EUR |
SLC4A1AP (Kanadaptin, Human Lung Cancer Oncogene 3 Protein, HLC-3, Kidney Anion Exchanger Adapter Protein, Solute Carrier Family 4 Anion Exchanger Member 1 Adapter Protein, HLC3) (MaxLight 405) |
|||
| MBS6192697-5x01mL | MyBiosource | 5x0.1mL | 3800 EUR |
SLC4A1AP (Kanadaptin, Human Lung Cancer Oncogene 3 Protein, HLC-3, Kidney Anion Exchanger Adapter Protein, Solute Carrier Family 4 Anion Exchanger Member 1 Adapter Protein, HLC3) (MaxLight 490) |
|||
| MBS6203372-01mL | MyBiosource | 0.1(mL | 875 EUR |
SLC4A1AP (Kanadaptin, Human Lung Cancer Oncogene 3 Protein, HLC-3, Kidney Anion Exchanger Adapter Protein, Solute Carrier Family 4 Anion Exchanger Member 1 Adapter Protein, HLC3) (MaxLight 490) |
|||
| MBS6203372-5x01mL | MyBiosource | 5x0.1mL | 3800 EUR |
SLC4A1AP (Kanadaptin, Human Lung Cancer Oncogene 3 Protein, HLC-3, Kidney Anion Exchanger Adapter Protein, Solute Carrier Family 4 Anion Exchanger Member 1 Adapter Protein, HLC3) (MaxLight 550) |
|||
| MBS6214047-01mL | MyBiosource | 0.1(mL | 875 EUR |
SLC4A1AP (Kanadaptin, Human Lung Cancer Oncogene 3 Protein, HLC-3, Kidney Anion Exchanger Adapter Protein, Solute Carrier Family 4 Anion Exchanger Member 1 Adapter Protein, HLC3) (MaxLight 550) |
|||
| MBS6214047-5x01mL | MyBiosource | 5x0.1mL | 3800 EUR |
SLC4A1AP (Kanadaptin, Human Lung Cancer Oncogene 3 Protein, HLC-3, Kidney Anion Exchanger Adapter Protein, Solute Carrier Family 4 Anion Exchanger Member 1 Adapter Protein, HLC3) (MaxLight 750) |
|||
| MBS6235397-01mL | MyBiosource | 0.1(mL | 875 EUR |
SLC4A1AP (Kanadaptin, Human Lung Cancer Oncogene 3 Protein, HLC-3, Kidney Anion Exchanger Adapter Protein, Solute Carrier Family 4 Anion Exchanger Member 1 Adapter Protein, HLC3) (MaxLight 750) |
|||
| MBS6235397-5x01mL | MyBiosource | 5x0.1mL | 3800 EUR |
STRADA (LYK5, STE20-related Kinase Adapter Protein alpha, STRAD alpha, STE20-related Adapter Protein, Serologically Defined Breast Cancer Antigen NY-BR-96, STRAD, FLJ90524) (MaxLight 650) |
|||
| MBS6223051-01mL | MyBiosource | 0.1(mL | 875 EUR |
STRADA (LYK5, STE20-related Kinase Adapter Protein alpha, STRAD alpha, STE20-related Adapter Protein, Serologically Defined Breast Cancer Antigen NY-BR-96, STRAD, FLJ90524) (MaxLight 650) |
|||
| MBS6223051-5x01mL | MyBiosource | 5x0.1mL | 3800 EUR |
STRADA (LYK5, STE20-related Kinase Adapter Protein alpha, STRAD alpha, STE20-related Adapter Protein, Serologically Defined Breast Cancer Antigen NY-BR-96, STRAD, FLJ90524) (MaxLight 490) |
|||
| MBS6201701-01mL | MyBiosource | 0.1(mL | 875 EUR |
STRADA (LYK5, STE20-related Kinase Adapter Protein alpha, STRAD alpha, STE20-related Adapter Protein, Serologically Defined Breast Cancer Antigen NY-BR-96, STRAD, FLJ90524) (MaxLight 490) |
|||
| MBS6201701-5x01mL | MyBiosource | 5x0.1mL | 3800 EUR |
STRADA (LYK5, STE20-related Kinase Adapter Protein alpha, STRAD alpha, STE20-related Adapter Protein, Serologically Defined Breast Cancer Antigen NY-BR-96, STRAD, FLJ90524) (MaxLight 405) |
|||
| MBS6191026-01mL | MyBiosource | 0.1(mL | 875 EUR |
STRADA (LYK5, STE20-related Kinase Adapter Protein alpha, STRAD alpha, STE20-related Adapter Protein, Serologically Defined Breast Cancer Antigen NY-BR-96, STRAD, FLJ90524) (MaxLight 405) |
|||
| MBS6191026-5x01mL | MyBiosource | 5x0.1mL | 3800 EUR |
STRADA (LYK5, STE20-related Kinase Adapter Protein alpha, STRAD alpha, STE20-related Adapter Protein, Serologically Defined Breast Cancer Antigen NY-BR-96, STRAD, FLJ90524) (MaxLight 550) |
|||
| MBS6212376-01mL | MyBiosource | 0.1(mL | 875 EUR |
STRADA (LYK5, STE20-related Kinase Adapter Protein alpha, STRAD alpha, STE20-related Adapter Protein, Serologically Defined Breast Cancer Antigen NY-BR-96, STRAD, FLJ90524) (MaxLight 550) |
|||
| MBS6212376-5x01mL | MyBiosource | 5x0.1mL | 3800 EUR |
STRADA (LYK5, STE20-related Kinase Adapter Protein alpha, STRAD alpha, STE20-related Adapter Protein, Serologically Defined Breast Cancer Antigen NY-BR-96, STRAD, FLJ90524) (MaxLight 750) |
|||
| MBS6233726-01mL | MyBiosource | 0.1(mL | 875 EUR |
STRADA (LYK5, STE20-related Kinase Adapter Protein alpha, STRAD alpha, STE20-related Adapter Protein, Serologically Defined Breast Cancer Antigen NY-BR-96, STRAD, FLJ90524) (MaxLight 750) |
|||
| MBS6233726-5x01mL | MyBiosource | 5x0.1mL | 3800 EUR |
0.2ml Adapter pack |
|||
| C1008-A2-9 | Benchmark Scientific | 1 PC | 475.15 EUR |
MM-Separator PCR strip adapter |
|||
| MD90003 | AMSBIO | 1 | 192 EUR |
Adapter Xk50 - EACH |
|||
| 28989880 | Scientific Laboratory Supplies | EACH | 591.3 EUR |
Flow Adapter - EACH |
|||
| GAS1324 | Scientific Laboratory Supplies | EACH | 114.75 EUR |
Biosart 100 Monitor Adapter - EACH |
|||
| 16424 | Scientific Laboratory Supplies | EACH | 43.2 EUR |
STE20-related Kinase Adapter Protein alpha, CT (STE20-related Kinase Adaptor alpha, STRAD alpha, STRADA, STRAD, FLJ90524, LYK5, NY-BR-96, PMSE, Serologically Defined Breast Cancer Antigen, STE20-related Adapter Protein, Stlk) (Biotin) |
|||
| MBS6501102-02mL | MyBiosource | 0.2mL | 980 EUR |
STE20-related Kinase Adapter Protein alpha, CT (STE20-related Kinase Adaptor alpha, STRAD alpha, STRADA, STRAD, FLJ90524, LYK5, NY-BR-96, PMSE, Serologically Defined Breast Cancer Antigen, STE20-related Adapter Protein, Stlk) (Biotin) |
|||
| MBS6501102-5x02mL | MyBiosource | 5x0.2mL | 4250 EUR |
STE20-related Kinase Adapter Protein alpha, NT (STE20-related Kinase Adaptor alpha, STRAD alpha, STRADA, STRAD, FLJ90524, LYK5, NY-BR-96, PMSE, Serologically Defined Breast Cancer Antigen, STE20-related Adapter Protein, Stlk) (Biotin) |
|||
| MBS6501113-02mL | MyBiosource | 0.2mL | 980 EUR |
STE20-related Kinase Adapter Protein alpha, NT (STE20-related Kinase Adaptor alpha, STRAD alpha, STRADA, STRAD, FLJ90524, LYK5, NY-BR-96, PMSE, Serologically Defined Breast Cancer Antigen, STE20-related Adapter Protein, Stlk) (Biotin) |
|||
| MBS6501113-5x02mL | MyBiosource | 5x0.2mL | 4250 EUR |
Biometra TRobot II Adapter Frame |
|||
| AJ846-070-924 | Westburg | each | 348.8 EUR |
Luer-Lock Adapter |
|||
| 54-119N | Genesee Scientific | 1 Adapter/Unit | 5.2 EUR |
Adapter Syringe - EACH |
|||
| DEN1010 | Scientific Laboratory Supplies | EACH | 63.45 EUR |
Adapter molecule crk |
|||
| AP81855 | SAB | 1mg | 2640 EUR |
Adapter molecule crk |
|||
| AP81873 | SAB | 1mg | 2640 EUR |
Adapter molecule crk |
|||
| AP82069 | SAB | 1mg | 2640 EUR |
Adapter molecule crk |
|||
| AP82231 | SAB | 1mg | 2640 EUR |
Adapter protein GRID. |
|||
| FP-0034 | Signosis | Each | Ask for price |
TADA2L, ID (TADA2A, TADA2L, Transcriptional adapter 2-alpha, Transcriptional adapter 2-like) (Biotin) |
|||
| MBS6353294-02mL | MyBiosource | 0.2mL | 980 EUR |
TADA2L, ID (TADA2A, TADA2L, Transcriptional adapter 2-alpha, Transcriptional adapter 2-like) (Biotin) |
|||
| MBS6353294-5x02mL | MyBiosource | 5x0.2mL | 4250 EUR |
STE20-Related Kinase Adapter Protein Alpha (STRADA) Antibody |
|||
| abx013673-100l | Abbexa | 100 µl | 43.75 EUR |
STE20-Related Kinase Adapter Protein Alpha (STRADA) Antibody |
|||
| 20-abx013673 | Abbexa |
|
|
Stacking Adapter - EACH |
|||
| 480158 | Scientific Laboratory Supplies | EACH | 255.15 EUR |
Adapter 80 - 120mL 2pcs - PK2 |
|||
| E5825723007 | Scientific Laboratory Supplies | PK2 | 233.55 EUR |
Extension Adapter - EACH |
|||
| EVA5034 | Scientific Laboratory Supplies | EACH | 59.4 EUR |
MM-Separator PCR strip adapter P |
|||
| MDMG0003 | AMSBIO | 1 | 192 EUR |
Ac Adapter UK - EACH |
|||
| F30705 | Scientific Laboratory Supplies | EACH | 93.15 EUR |
200 µl Tip Adapter |
|||
| VAS-E-35 | Phoenix instrument | each | 19 EUR |
1000 µl Tip Adapter |
|||
| VAS-E-36 | Phoenix instrument | each | 31 EUR |
200 µl Tip Adapter |
|||
| VAS-E35 | PHOENIX PEPTIDE | 200 µl | 19 EUR |
1000 µl Tip Adapter |
|||
| VAS-E36 | PHOENIX PEPTIDE | 1000 µl | 31 EUR |
Adapter 17.5 x 75-100mm - EACH |
|||
| E5920708007 | Scientific Laboratory Supplies | EACH | 359.1 EUR |
PD-10 Spin Adapter |
|||
| 60506-5Pieces | AAT Bioquest | 5 Pieces | 79 EUR |
PD-10 Spin Adapter |
|||
| 60507-10Pieces | AAT Bioquest | 10 Pieces | 99 EUR |
STE20-related Kinase Adapter Protein alpha, NT (STE20-related Kinase Adaptor alpha, STRAD alpha, STRADA, STRAD, FLJ90524, LYK5, NY-BR-96, PMSE, Serologically Defined Breast Cancer Antigen, STE20-related Adapter Protein, Stlk) |
|||
| MBS628728-02mL | MyBiosource | 0.2mL | 695 EUR |
STE20-related Kinase Adapter Protein alpha, NT (STE20-related Kinase Adaptor alpha, STRAD alpha, STRADA, STRAD, FLJ90524, LYK5, NY-BR-96, PMSE, Serologically Defined Breast Cancer Antigen, STE20-related Adapter Protein, Stlk) |
|||
| MBS628728-5x02mL | MyBiosource | 5x0.2mL | 2975 EUR |
STE20-related Kinase Adapter Protein alpha, CT (STE20-related Kinase Adaptor alpha, STRAD alpha, STRADA, STRAD, FLJ90524, LYK5, NY-BR-96, PMSE, Serologically Defined Breast Cancer Antigen, STE20-related Adapter Protein, Stlk) |
|||
| MBS627023-02mL | MyBiosource | 0.2mL | 695 EUR |
STE20-related Kinase Adapter Protein alpha, CT (STE20-related Kinase Adaptor alpha, STRAD alpha, STRADA, STRAD, FLJ90524, LYK5, NY-BR-96, PMSE, Serologically Defined Breast Cancer Antigen, STE20-related Adapter Protein, Stlk) |
|||
| MBS627023-5x02mL | MyBiosource | 5x0.2mL | 2975 EUR |
Rat STE20 related kinase adapter protein Alpha (STRADA) ELISA kit |
|||
| E01A16710 | BlueGene | 96T | 700 EUR |
Pig STE20 related kinase adapter protein Alpha (STRADA) ELISA kit |
|||
| E07S0416-192T | BlueGene | 192 tests | 1524 EUR |
Pig STE20 related kinase adapter protein Alpha (STRADA) ELISA kit |
|||
| E07S0416-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
Pig STE20 related kinase adapter protein Alpha (STRADA) ELISA kit |
|||
| E07S0416-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
Rat STE20 related kinase adapter protein Alpha (STRADA) ELISA kit |
|||
| E02S0416-192T | BlueGene | 192 tests | 1524 EUR |
Rat STE20 related kinase adapter protein Alpha (STRADA) ELISA kit |
|||
| E02S0416-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
Rat STE20 related kinase adapter protein Alpha (STRADA) ELISA kit |
|||
| E02S0416-48wellsplate | BlueGene | 48 wells plate | 280 EUR |
Rat STE20 related kinase adapter protein Alpha (STRADA) ELISA kit |
|||
| E02S0416-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
Rat STE20 related kinase adapter protein Alpha (STRADA) ELISA kit |
|||
| E02S0416-96wellsplate | BlueGene | 96 wells plate | 405 EUR |
Dog STE20 related kinase adapter protein Alpha (STRADA) ELISA kit |
|||
| E08S0416-192T | BlueGene | 192 tests | 1524 EUR |
Dog STE20 related kinase adapter protein Alpha (STRADA) ELISA kit |
|||
| E08S0416-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
Dog STE20 related kinase adapter protein Alpha (STRADA) ELISA kit |
|||
| E08S0416-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
DIN plug adapter - EACH |
|||
| PHM1251 | Scientific Laboratory Supplies | EACH | 371.61 EUR |
BNC plug adapter - EACH |
|||
| PHM1252 | Scientific Laboratory Supplies | EACH | 371.61 EUR |
Recombinant Staphylococcus aureus (strain MW2) Adapter protein MecA |
|||
| MBS1000312-002mgEColi | MyBiosource | 0.02mg(E-Coli) | 375 EUR |
Recombinant Staphylococcus aureus (strain MW2) Adapter protein MecA |
|||
| MBS1000312-01mgEColi | MyBiosource | 0.1mg(E-Coli) | 635 EUR |
Recombinant Staphylococcus aureus (strain MW2) Adapter protein MecA |
|||
| MBS1000312-1mgEColi | MyBiosource | 1mg(E-Coli) | 1925 EUR |
Recombinant Staphylococcus aureus (strain MW2) Adapter protein MecA |
|||
| MBS1000312-5x1mgEColi | MyBiosource | 5x1mg(E-Coli) | 8405 EUR |
STE20-related Kinase Adapter Protein alpha, CT (STE20-related Kinase Adaptor alpha, STRAD alpha, STRADA, STRAD, FLJ90524, LYK5, NY-BR-96, PMSE, Serologically Defined Breast Cancer Antigen, STE20-related Adapter Protein, Stlk) (AP) |
|||
| MBS6501100-02mL | MyBiosource | 0.2mL | 980 EUR |
STE20-related Kinase Adapter Protein alpha, CT (STE20-related Kinase Adaptor alpha, STRAD alpha, STRADA, STRAD, FLJ90524, LYK5, NY-BR-96, PMSE, Serologically Defined Breast Cancer Antigen, STE20-related Adapter Protein, Stlk) (AP) |
|||
| MBS6501100-5x02mL | MyBiosource | 5x0.2mL | 4250 EUR |
STE20-related Kinase Adapter Protein alpha, CT (STE20-related Kinase Adaptor alpha, STRAD alpha, STRADA, STRAD, FLJ90524, LYK5, NY-BR-96, PMSE, Serologically Defined Breast Cancer Antigen, STE20-related Adapter Protein, Stlk) (PE) |
|||
| MBS6501110-02mL | MyBiosource | 0.2mL | 980 EUR |
STE20-related Kinase Adapter Protein alpha, CT (STE20-related Kinase Adaptor alpha, STRAD alpha, STRADA, STRAD, FLJ90524, LYK5, NY-BR-96, PMSE, Serologically Defined Breast Cancer Antigen, STE20-related Adapter Protein, Stlk) (PE) |
|||
| MBS6501110-5x02mL | MyBiosource | 5x0.2mL | 4250 EUR |
STE20-related Kinase Adapter Protein alpha, NT (STE20-related Kinase Adaptor alpha, STRAD alpha, STRADA, STRAD, FLJ90524, LYK5, NY-BR-96, PMSE, Serologically Defined Breast Cancer Antigen, STE20-related Adapter Protein, Stlk) (AP) |
|||
| MBS6501111-02mL | MyBiosource | 0.2mL | 980 EUR |
STE20-related Kinase Adapter Protein alpha, NT (STE20-related Kinase Adaptor alpha, STRAD alpha, STRADA, STRAD, FLJ90524, LYK5, NY-BR-96, PMSE, Serologically Defined Breast Cancer Antigen, STE20-related Adapter Protein, Stlk) (AP) |
|||
| MBS6501111-5x02mL | MyBiosource | 5x0.2mL | 4250 EUR |
STE20-related Kinase Adapter Protein alpha, NT (STE20-related Kinase Adaptor alpha, STRAD alpha, STRADA, STRAD, FLJ90524, LYK5, NY-BR-96, PMSE, Serologically Defined Breast Cancer Antigen, STE20-related Adapter Protein, Stlk) (PE) |
|||
| MBS6501121-02mL | MyBiosource | 0.2mL | 980 EUR |
STE20-related Kinase Adapter Protein alpha, NT (STE20-related Kinase Adaptor alpha, STRAD alpha, STRADA, STRAD, FLJ90524, LYK5, NY-BR-96, PMSE, Serologically Defined Breast Cancer Antigen, STE20-related Adapter Protein, Stlk) (PE) |
|||
| MBS6501121-5x02mL | MyBiosource | 5x0.2mL | 4250 EUR |
Goat STE20 related kinase adapter protein Alpha (STRADA) ELISA kit |
|||
| E01A51611 | BlueGene | 96T | 700 EUR |
Goat STE20 related kinase adapter protein Alpha (STRADA) ELISA kit |
|||
| E06S0416-192T | BlueGene | 192 tests | 1524 EUR |
Goat STE20 related kinase adapter protein Alpha (STRADA) ELISA kit |
|||
| E06S0416-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
Goat STE20 related kinase adapter protein Alpha (STRADA) ELISA kit |
|||
| E06S0416-48wellsplate | BlueGene | 48 wells plate | 280 EUR |
Goat STE20 related kinase adapter protein Alpha (STRADA) ELISA kit |
|||
| E06S0416-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
Goat STE20 related kinase adapter protein Alpha (STRADA) ELISA kit |
|||
| E06S0416-96wellsplate | BlueGene | 96 wells plate | 405 EUR |
Goat STE20 Related Kinase Adapter Protein Alpha (STRADA) ELISA Kit |
|||
| MBS7260024-10x96StripWells | MyBiosource | 10x96-Strip-Wells | 5685 EUR |
Goat STE20 Related Kinase Adapter Protein Alpha (STRADA) ELISA Kit |
|||
| MBS7260024-48StripWells | MyBiosource | 48-Strip-Wells | 485 EUR |
Goat STE20 Related Kinase Adapter Protein Alpha (STRADA) ELISA Kit |
|||
| MBS7260024-5x96StripWells | MyBiosource | 5x96-Strip-Wells | 3020 EUR |
qPCR-Assay: Für die Leistung des qPCR-Assays wurde ein Standardassay mit Lambda-DNA (Promega) und KAPA SYBR Fast qPCR Mastermix (KAPA Biosystems) verwendet. Drei unabhängige qPCR-Assays über sechs Konzentrationsprotokolle der Lambda-DNA-Matrize (101-106) und 3 Wiederholungen für jede Konzentration wurden durchgeführt. Die Kompatibilität wurde basierend auf den folgenden qPCR-Reaktionsparametern bewertet: Ct-Mittelwert, Ct-SD, CV, Schmelzkurvenanalyse (Daten nicht vorgestellt).