Bacteriële celculturen in een lab-on-a-disc: een eenvoudig en veelzijdig

Bacteriële celculturen in een lab-on-a-disc: een eenvoudig en veelzijdig

Bacteriële celculturen in een lab-on-a-disc: een eenvoudig en veelzijdig apparaat voor het kwantificeren van de werkzaamheid van antibiotica

Pathogene bacteriële biofilms zullen levensbedreigend zijn, de hoge kwaliteit van leven van de getroffen persoon aanzienlijk verlagen en een aanzienlijke belasting voor de gezondheidszorg vormen. Huidige strategieën voor analyse van antibacteriële therapieën in klinieken en in vitro methoden die worden gebruikt bij de groei en screening van geneesmiddelen, vergemakkelijken beide de vorming van biofilm niet of zijn lastig te functioneren, willen enorme reagensvolumes en zijn duur, waardoor hun bruikbaarheid beperkt is.

Om met deze punten om te gaan, presenteert dit werk het evenement van een stevig in vitro celtraditieplatform dat geschikt is voor confocale microscopie.

Het platform dat is gevormd als een compact disc, faciliteert een lange bacteriële traditie zonder externe pompen en slangen en zal een aantal dagen worden gebruikt zonder extra vloeistof. Pseudomonas aeruginosa biofilm wordt bijvoorbeeld gekweekt uit afzonderlijke cellen en het is bewezen dat:

1) het platform levert reproduceerbare en betrouwbare resultaten; 2) vooruitgang is afhankelijk van bewegingslading en de samenstelling van het voortgangsmedium; en drie) de doeltreffendheid van het antibioticum zal afhangen van de gevormde biofilm. Dit platform maakt de voortgang, kwantificering en remedie van biofilm mogelijk zoals in een traditionele bewegingsopstelling, terwijl de apparaatbarrière van lab-on-chip-methoden wordt verkleind. Het biedt een gebruiksvriendelijke, redelijk geprijsde mogelijkheid voor eindklanten die werken met celkweek in relatie tot b.v. diagnostiek en drugsscreening.

skinsandbones
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Bead Sample Pack - 50mL Set

BSP-50B2 1pack
EUR 404
Description: Bead sample pack for Bullet Blender 50mL tube models. Includes 20mL of: ZROB05, ZROB20, SSB02, SSB14B, SSB48, SSB60, SSUFO35 and SSUFO56.

Bead Sample Pack - 5E Set

BSP-5E 1pack
EUR 252
Description: Bead sample pack for Bullet Blender 5E models. Includes 10mL of: ZROB015, ZROB05, ZROB20, SSB02, SSB14B, SSB32, SSB48, SSUFO35 and SSUFO56.

Bead Sample Pack - 5M Set

BSP-5Y 1pack
EUR 241
Description: Bead sample pack for Bullet Blender 5mL Storm models. Includes 10mL of: ZROB015, ZROB05, ZROB20, SSB02, SSB14B, SSB32, SSB60, SSB110 and SSUFO35.

Bead Sample Pack - Full Set

BSP-ALL2 1pack
EUR 384
Description: Bead sample pack inlcudes sampling of all 20 bead types in 10mL tubes.

Bead Sample Pack - Microcentrifuge Set

BSP-MC2 1pack
EUR 232
Description: Bead sample pack for Bullet Blender 1.5mL tube models. Includes 10mL of: ZROB015, ZROB05, ZROB10, ZROB20, SSB02, SSB14B, SSB32 and SSUFO35.

Bead Sample Pack - Organ Tissues

BSP-OT3 1pack
EUR 235
Description: Bead sample pack for homogenization of organ tissues. Includes 10mL of: ZROB05, ZROB10, ZROB20, SSB14B, SSB32 and SSUFO35.

All Sample Diluents Sample Pack

KF17355 3X100 ml
EUR 272

Red Blood Cell Sample Pack

88R-1050 6 x 2 ml
EUR 403
Description: Horse, Mouse, Rabbit, Sheep, Bovine and Chicken glutaraldehyde stabilized blood cell samples

Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome) sample

P100EZ-sample NULL
EUR 0

TMB Peroxidase Substrate SAMPLE PACK

42R-TB101 3 x 100 ml
EUR 300
Description: 100mL of 3 different TMB Peroxidase Substrates ready to use in immunoassays: Ultra sensitivite, Highly Sensitive, and Slow Acting.

3 Blocking Buffer Sample Pack

KF17340 3 x 100 ml
EUR 205

All Assay Diluents Sample Pack

KF17351 4X100 ml
EUR 272

Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome) sample

P101EZ--sample NULL
EUR 0

Water, 0.1um Filtered, cell cultures tested

W0900-050 500 ml
EUR 68

Water, 0.1um Filtered, cell cultures tested

W0900-310 10x500 ml
EUR 169

Water, 0.1um Filtered, cell cultures tested

W0900-350 50x500 ml
EUR 523

Micro Bead Sterlizer

B1201-E 1 PC
EUR 542.7
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Micro Bead Sterlizer

B1202-E 1 PC
EUR 84.14
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Refill Glass Beads

B1201-BEAD 1 PC
EUR 117.78
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Column Packing Kit

PACK-KIT 1pack
EUR 1035
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

Sample Diluent

I094 1000 ml
EUR 519
  • Product category: Buffer solution
Description: Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

Sample Diluent

I094-100 100 ml
EUR 211
  • Product category: Buffer solution
Description: Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

Sample Diluent

I094-500 500 ml
EUR 373
  • Product category: Buffer solution
Description: Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

Bangs Lab Bead Solution

SOLN1-1000 1000 ML
EUR 155.06
Description: Bangs Lab Bead Solution is ready-to-use solution and is suitable for dilution and/or storage of plain, dyed, or functionalized polymer microspheres

Bangs Lab Bead Solution

SOLN1-2000 2000 ML
EUR 212.7
Description: Bangs Lab Bead Solution is ready-to-use solution and is suitable for dilution and/or storage of plain, dyed, or functionalized polymer microspheres

Bangs Lab Bead Solution

SOLN1-500 500 ML
EUR 98.51
Description: Bangs Lab Bead Solution is ready-to-use solution and is suitable for dilution and/or storage of plain, dyed, or functionalized polymer microspheres

Adapter pack

C1005-AC2 1 PC
EUR 98.5
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Adapter pack

C3100-ADP 1 PC
EUR 164.18
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EZ Pack

A2501 1 PC
EUR 365.66
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EZ Pack

A2505 1 PC
EUR 183.03
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Long-term Cell Tracer 580 (Green) sample

P710GS NULL
EUR 0

Long-term Cell Tracer 580 (Yellow) sample

P710YS NULL
EUR 0

Sample diluent buffer

AR1106-1 30ml
EUR 91

Rotavirus Positive Sample

DAG-H10377 1 ml dry ice
EUR 1105

RSV Positive Sample

DAG-H10378 1 ml dry ice
EUR 1105

DNA Sample Diluent

D006-100 100 ml
EUR 373
  • Product category: Buffer solution
Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

DNA Sample Diluent

D006-1000 1000 ml
EUR 1167
  • Product category: Buffer solution
Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

DNA Sample Diluent

D006-500 500 ml
EUR 726
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Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

Sample Diluent Buffer

abx296001-20ml 20 ml
EUR 91
  • Shipped within 5-10 working days.

BSA Sample Diluent

F031A 1000 ml
EUR 488
  • Product category: Buffer solution
Description: BSA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

BSA Sample Diluent

F031A-100 100 ml
EUR 195
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Description: BSA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

BSA Sample Diluent

F031A-500 500 ml
EUR 411
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Description: BSA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

Sample Denaturing Buffer

F054 12 ml
EUR 195
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Sample Treatment Buffer

F175 1000 ml
EUR 411
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Sample Treatment Buffer

F175-100 100 ml
EUR 219
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Sample Treatment Plate

F402 1x 96 wells plate
EUR 103
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ELISA Sample Diluent

ESD125 125 ml
EUR 80

ELISA Sample Diluent

ESD500 500 ml
EUR 109

ELISA Sample Diluent

ESD999 1000 ml
EUR 157

Sample Diluent Buffer

I028-100 100 ml
EUR 211
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Description: Sample Diluent Buffer by Cygnus Technologies is available in Europe via Gentaur.

Sample Diluent Buffer

I028-1000 1000 ml
EUR 519
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Description: Sample Diluent Buffer by Cygnus Technologies is available in Europe via Gentaur.

Sample Diluent Buffer

I028-500 500 ml
EUR 373
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HEPES Sample Diluent

I050 1000 ml
EUR 527
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Description: HEPES Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

HEPES Sample Diluent

I050-100 100 ml
EUR 219
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Description: HEPES Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

HEPES Sample Diluent

I050-500 500 ml
EUR 334
  • Product category: Buffer solution
Description: HEPES Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

Sample Diluent, 60ML

X028-60ML 60ML
EUR 143

Protein A Tell-Tale Gold Ribbon Sample Pack, 3 x (1 cm x 30 cm)

TTGA-SP3-B090 3 x (1 cm x 30 cm)
EUR 227.67
  • Product Category: Gold Ribbon
Description: The pack contains polyester ribbon, which is soaked with 0.13 O.D./cm2 concentration with Protein G conjugated to gold nanoparticles.

Protein G Tell-Tale Gold Ribbon Sample Pack, 3 x (1 cm x 30 cm)

TTGG-SP3-B090 3 x (1 cm x 30 cm)
EUR 227.67
  • Product Category: Gold Ribbon
Description: The pack contains polyester ribbon, which is soaked with 3 different concentrations with Protein G conjugated to gold nanoparticles.

DEAE Rapid Run Agarose Bead

DEAERR-25 25 ml
EUR 102

DEAE Rapid Run Agarose Bead

DEAERR-300 300 ml
EUR 492

CM Rapid Run Agarose Bead

CMRR-25 25 ml
EUR 102

CM Rapid Run Agarose Bead

CMRR-300 300 ml
EUR 492

SP Rapid Run Agarose Bead

SPRR-25 25 ml
EUR 102

SP Rapid Run Agarose Bead

SPRR-300 300 ml
EUR 492

Protein A/G Tell-Tale Gold Ribbon Sample Pack, 3 x (1 cm x 30 cm)

TTGAG-SP3-B090 3 x (1 cm x 30 cm)
EUR 227.67
  • Product Category: Gold Ribbon
Description: The pack contains polyester ribbon, which is soaked with 3 different concentrations with Protein A and G conjugated to gold nanoparticles.

Replacement generator pack

D1000-M5 1 PC
EUR 531.32
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Replacement generator pack

D1000-M7 1 PC
EUR 415.61
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0.2ml Adapter pack

C1008-A2-6 1 PC
EUR 84.94
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

0.2ml Adapter pack

C1008-A2-9 1 PC
EUR 395.96
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

0.5ml Adapter pack

C1008-A5-6 1 PC
EUR 80.95
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

0.5ml Adapter pack

C1008-A5-8 1 PC
EUR 84.94
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

mini cooling pack

EVS1100-COOL ea
EUR 48

maxi cooling pack

EVS1300-COOL ea
EUR 82

Safe-Pack™

G108-P G108-WGR
EUR 104

Spatulas (Large Pack)

SPAT1 50pack
EUR 301
Description: Individually wrapped spatulas. RNase/DNase free, non-pyrogenic, antistatic, sterile, plastic. Pack of 50.

Spatulas (Small Pack)

SPAT2 10pack
EUR 133
Description: Individually wrapped spatulas. RNase/DNase free, non-pyrogenic, antistatic, sterile, plastic. Pack of 10.

CometAssay HT Sample Kit

4252-040-K 40 Tests
EUR 428

Deproteinizing Sample Preparation Kit

55R-1542 200 assays
EUR 450
Description: Deproteinizing Sample Preparation Kit for use in the research laboratory

Salivary Sample Collection Kit

55R-IB76305 500 tests
EUR 301
Description: Salivary sample collection kit for the detection of free Salivary proteins and enzymes in the research laboratory

comb 10 sample, 0.8mm

EVS3100-C10-0.8 ea
EUR 59

comb 10 sample, 1.5mm

EVS3100-C10-1.5 ea
EUR 59

comb 12 sample, 0.8mm

EVS3100-C12-0.8 ea
EUR 59

comb 12 sample, 1.5mm

EVS3100-C12-1.5 ea
EUR 59

comb 6 sample, 0.8mm

EVS3100-C6-0.8 ea
EUR 59

comb 6 sample, 1.5mm

EVS3100-C6-1.5 ea
EUR 59

comb 8 sample, 0.8mm

EVS3100-C8-0.8 ea
EUR 59

comb 8 sample, 1.5mm

EVS3100-C8-1.5 ea
EUR 59

comb 10 sample, 0.8mm

EVS3200-C10-0.8 ea
EUR 65

comb 10 sample, 1.5mm

EVS3200-C10-1.5 ea
EUR 65

comb 15 sample, 0.8mm

EVS3200-C15-0.8 ea
EUR 65

comb 15 sample, 1.5mm

EVS3200-C15-1.5 ea
EUR 65

comb 20 sample, 0.8mm

EVS3200-C20-0.8 ea
EUR 65

comb 20 sample, 1.5mm

EVS3200-C20-1.5 ea
EUR 65

comb 25 sample, 0.8mm

EVS3200-C25-0.8 ea
EUR 65

comb 25 sample, 1.5mm

EVS3200-C25-1.5 ea
EUR 65

comb 10 sample, 0.8mm

EVS3300-C10-0.8 ea
EUR 65

comb 10 sample, 1.5mm

EVS3300-C10-1.5 ea
EUR 65

comb 15 sample, 0.8mm

EVS3300-C15-0.8 ea
EUR 65

comb 15 sample, 1.5mm

EVS3300-C15-1.5 ea
EUR 65

comb 20 sample, 0.8mm

EVS3300-C20-0.8 ea
EUR 65

comb 20 sample, 1.5mm

EVS3300-C20-1.5 ea
EUR 65

comb 25 sample, 0.8mm

EVS3300-C25-0.8 ea
EUR 65

comb 25 sample, 1.5mm

EVS3300-C25-1.5 ea
EUR 65

SP2/0 Sample Diluent

F183A 1000 ml
EUR 835
  • Product category: Buffer solution
Description: SP2/0 Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

SP2/0 Sample Diluent

F183A-100 100 ml
EUR 257
  • Product category: Buffer solution
Description: SP2/0 Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

SP2/0 Sample Diluent

F183A-500 500 ml
EUR 488
  • Product category: Buffer solution
Description: SP2/0 Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

Goat IgG Sample Diluent

F213A 1000 ml
EUR 565
  • Product category: Buffer solution
Description: Goat IgG Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

Goat IgG Sample Diluent

F213A-100 100 ml
EUR 257
  • Product category: Buffer solution
Description: Goat IgG Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

Goat IgG Sample Diluent

F213A-500 500 ml
EUR 411
  • Product category: Buffer solution
Description: Goat IgG Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

NS/0 Sample Diluent

F223A 1000 ml
EUR 835
  • Product category: Buffer solution
Description: NS/0 Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

NS/0 Sample Diluent

F223A-100 100 ml
EUR 257
  • Product category: Buffer solution
Description: NS/0 Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

NS/0 Sample Diluent

F223A-500 500 ml
EUR 488
  • Product category: Buffer solution
Description: NS/0 Sample Diluent by Cygnus Technologies is available in Europe via Gentaur.

A549 Sample Diluent, 100mL

F233A-100 100 ml
EUR 257
  • Product category: Buffer solution
Description: A549 Sample Diluent, 100mL by Cygnus Technologies is available in Europe via Gentaur.

A549 Sample Diluent, 500mL

F233A-500 500 ml
EUR 411
  • Product category: Buffer solution
Description: A549 Sample Diluent, 500mL by Cygnus Technologies is available in Europe via Gentaur.

Neptune Sample Diluent-SD3

KF17354 500 ml
EUR 244

Deproteinizing Sample Preparation Kit

K808-200
EUR 338

Deproteinizing Sample Preparation Kit

K2184-200 200 assays
EUR 321

ProtoGel Sample Prep Kit

NAT1248 EACH
EUR 186

RoomTemp Sample Lysis Kit

P073-01 250 rxn (5 ml)
EUR 212

RoomTemp Sample Lysis Kit

P073-02 1000 rxn ( 20 ml)
EUR 473

RoomTemp Sample Lysis Kit

P073-03 5000 rxn (100 ml)
EUR 1400

Inactivated ADV Positive Sample

VAng-Lsx0001-inquire inquire Ask for price
Description: ADV Positive Sample, natural antigen.

Hemoglobin Sample Diluent, 50ML

X052-50ML 50ML
EUR 153

Sample Diluent (Conc.), 12ML

X074-12ML 12ML
EUR 123

Sample Diluent (Conc.), 60ML

X074-60ML 60ML
EUR 143

Recombinant RSV Positive Sample

VAng-Lsx0452-inquire inquire Ask for price
Description: RSV Positive Sample.

Coronavirus Sample Preparation Solution

M1461-50 50 ml Ask for price

Anti-DDK(FLAG) Agarose bead Conjugated

D4501-001 1ml
EUR 550

Anti-DDK(FLAG) Agarose bead Conjugated

D4501-005 5ml
EUR 1373

Nickel NTA Magnetic Agarose Bead (5%)

MagNTANi5-10 10ml suspension
EUR 284

Nickel NTA Magnetic Agarose Bead (5%)

MagNTANi5-2 2ml suspension
EUR 99

Nickel NTA Magnetic Agarose Bead (5%)

MagNTANi5-5 5ml suspension
EUR 174

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric

CBA-135 96 assays
EUR 821
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric

CBA-135-5 5 x 96 assays
EUR 3356
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric

CBA-140 96 assays
EUR 856
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric

CBA-140-5 5 x 96 assays
EUR 3483
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

293AD Cell Line

AD-100 1 vial
EUR 461
Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area.

293AAV Cell Line

AAV-100 1 vial
EUR 508
Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area.

293LTV Cell Line

LTV-100 1 vial
EUR 508
Description: The 293LTV Cell Line is derived from the parental 293 cells but selected for attributes that increase lentiviral production, including fimrer attachment and larger surface area.

293RTV Cell Line

RV-100 1 vial
EUR 508
Description: The 293RTV Cell Line is derived from the parental 293 cells but selected for attributes that increase retroviral production, including fimrer attachment and larger surface area.

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

CBA-325 96 assays
EUR 856
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

CBA-325-5 5 x 96 assays
EUR 3361
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

ELISA Starter Pack Kit

55R-E101PK 1 kit
EUR 563
Description: ELISA reagent tool kit for use as a starter pack for researchers

TBS Blocking Buffer Pack

AR0143 200mL/pack
EUR 65

TBS Wash Buffer Pack

AR0144 500mL/pack
EUR 60

PBS Wash Buffer Pack

AR1155 500mL/pack
EUR 65

cool-pack and platfom

EHS1100-COOL ea
EUR 90

cool-pack and platfom

EHS1200-COOL ea
EUR 107

cool-pack and platfom

EHS1300-COOL ea
EUR 124

cool-pack and platfom

EHS1400-COOL ea
EUR 124

cool-pack and platfom

EHS1500-COOL ea
EUR 149

Measuring Spoon (Large Pack)

SPN1 200pack
EUR 479
Description: Individually wrapped 1.2mL spoons. Sterile. Pack of 200.

Measuring Spoon (Small Pack)

SPN2 20pack
EUR 130
Description: Individually wrapped 1.2mL spoons. Sterile. Pack of 20.

MDR Sampler Pack antibody

STJ16100969 1 pack
EUR 666

TruStrip Sample Transfer Strips, 5-ul, 50/Pk (without sample tracking dye)

STS-05-50 1 Pk Ask for price

TruStrip Sample Transfer Strips, 100-ul, 50/Pk (without sample tracking dye)

STS-100-50 1 Pk Ask for price

TruStrip Sample Transfer Strips, 15-ul, 50/Pk (without sample tracking dye)

STS-15-50 1 Pk Ask for price

TruStrip Sample Transfer Strips, 200-ul, 50/Pk (without sample tracking dye)

STS-200-50 1 Pk Ask for price

TruStrip Sample Transfer Strips, 25-ul, 50/Pk (without sample tracking dye)

STS-25-50 1 Pk Ask for price

TruStrip Sample Transfer Strips, 400-ul, 50/Pk (without sample tracking dye)

STS-400-50 1 Pk Ask for price

TruStrip Sample Transfer Strips, 50-ul, 50/Pk (without sample tracking dye)

STS-50-50 1 Pk Ask for price

TruStrip Sample Transfer Strips, 800-ul, 50/Pk (without sample tracking dye)

STS-800-50 1 Pk Ask for price

TruStrip Sample Transfer Strips, 5-ul, 50/Pk (with sample tracking dye)

STSD-05-50 1 Pk Ask for price

TruStrip Sample Transfer Strips, 100-ul, 50/Pk (with sample tracking dye)

STSD-100-50 1 Pk Ask for price

TruStrip Sample Transfer Strips, 15-ul, 50/Pk (with sample tracking dye)

STSD-15-50 1 Pk Ask for price

TruStrip Sample Transfer Strips, 200-ul, 50/Pk (with sample tracking dye)

STSD-200-50 1 Pk Ask for price

TruStrip Sample Transfer Strips, 25-ul, 50/Pk (with sample tracking dye)

STSD-25-50 1 Pk Ask for price

TruStrip Sample Transfer Strips, 400-ul, 50/Pk (with sample tracking dye)

STSD-400-50 1 Pk Ask for price

Doorzichtig papier van microkristallijne cellulose / polyvinylalcohol als nieuw platform voor 3D-celcultuur

Meerlagig en gestapeld cellulosepapier is naar voren gekomen als een veelbelovend platform voor het bouwen van een driedimensionale (3D) celtraditie, dankzij de lage prijs, goede biocompatibiliteit en overmatige porositeit. Desalniettemin maakt de slechte, milde transmissie het moeilijk om celgedrag (bijv. Voortgang en proliferatie) onmiddellijk en duidelijk te volgen op het op papier gebaseerde platform met behulp van een optische microscoop.

Voor dit werk hebben we een helder microkristallijn cellulose / polyvinylalcohol (MCC / PVA) papier ontwikkeld met onregelmatige poriën via oplossen en regeneratie van microkristallijne nanocellulose (MCC), toevoeging van porogeen reagens (NaCl) en vervolgens onderdompelen in PVA-opties. Het heldere MCC-papier vertoont overmatige porositeit (maar liefst 90%), instelbare poriënmeting (tussen 23 μm en 46 μm) en enorme dikte (van 315 μm tot 436 μm) en overmatige milde transmissie onder water (> 95%).

• Via een aanvullende modificatie van helder MCC-papier met PVA, vertoont het verkregen heldere MCC / PVA-papier verbeterde mechanische eigenschappen (droge en vochtige sterkte), goede hydrofiliciteit (met een contacthoek van 70,8 °) en verbeterde biocompatibiliteit (levensvatbaarheid van de cellen zelfs 90 %). Door een aantal lagen van het heldere MCC / PVA-papier te stapelen en ontstapelen, is het gebruikt voor elk 2D- en 3D-celtraditieplatform.

• Het heldere MCC / PVA-papier onder water maakt elke directe verklaring van celmorfologie mogelijk met een optische microscoop door middel van blote ogen en fluorescentiemicroscoop na kleuring. We stellen ons voor dat het ontwikkelde, heldere MCC / PVA-papier een mooi potentieel biedt voor toekomstige doeleinden op tal van bioanalytische en biomedische gebieden, vergelijkbaar met het screenen van geneesmiddelen, tissue engineering en organ-on-chips.

Natuurequivalente verbindingen en natuurlijke zuren verbeteren en stoppen de schade veroorzaakt door een ontstekingsprobleem in Caco-2 celcultuur

Bioactieve verbindingen, vergelijkbaar met natuurlijke zuren (OA) en natuuridentieke verbindingen (NIC), kunnen een baan uitoefenen binnen de veiligheid van de werking van het darmslijmvlies, die toe te schrijven is aan hun organische eigenschappen.

Het doel van dit onderzoek was om de functie te begrijpen van twee OA (citroenzuur en sorbinezuur) en een paar NIC’s (thymol en vanilline), alleen of gemengd in een mix (OA + NIC), op de prestatie van de darmbarrière, beide gedurende homeostatische situatie of tijdens een ontstekingsprobleem uitgevoerd met pro-inflammatoire cytokines en lipopolysacchariden (LPS). Het onderzoek werd uitgevoerd op de menselijke epitheliale cellijn Caco-2, een bekende mannequin van de intestinale epitheliale barrière.

De resultaten bevestigden hoe OA en NIC alleen de transepitheliale elektrische weerstand (TEER) en mRNA-reeksen van tight junction (TJ) -delen kunnen verbeteren, maar OA + NIC bevestigde een sterkere werkzaamheid in vergelijking met de afzonderlijke moleculen.

Wanneer zich een ontstekingsprobleem voordeed, was OA + NIC-mix in een positie om elk letsel te verbeteren en te stoppen als gevolg van de pro-inflammatoire stimulus, waardoor de daling van TEER werd verminderd of gestopt en de TJ-mRNA-expressie werd versterkt. De informatie ondersteunt de functie van OA + NIC bij het moduleren van de darmstaaf

Realtime polymerasekettingreactie (realtime PCR)

Het is ook bekend als een kwantitatieve polymerasekettingreactie. Tijdens de PCR controleert het de toepassing van een gericht DNA. Omdat het een laboratoriumtechniek van moleculaire biologie is die is gebaseerd op de polymerasekettingreactie. Real-time PCR wordt kwantitatief op twee manieren gebruikt, d.w.z. semi-kwantitatief en als de hoeveelheid DNA lager of hoger is, zal het semi-kwantitatieve real-time PCR zijn.

Het is de methode die helpt om de hoeveelheid PR-product te bepalen. Een kwantitatieve polymerasekettingreactie wordt ook wel real-time PCR genoemd. Iedereen die de afgelopen dagen heeft geprobeerd kwantisatie uit te voeren, herinnert zich experimenten waarin nucleïnezurextracten werden uitgevoerd om tot een verdunning te komen die een bereik van de test zou zijn. Dit heeft geleid tot een ongebruikelijk algoritme met betrekking tot het falen van controles voor kwalitatieve nucleïnezuuramplificatietesten.

Methoden van PCR

• Niet-specifieke fluorescerende kleurstoffen.
Het is een kleurstof die intercaleert met elk van het dubbelstrengs DNA.
• Sequentiespecifieke DNA-sondes
Het is gelabeld met een fluorescerende reporter, die de detectie alleen mogelijk maakt na hybridisatie van de sonde met zijn complementaire sequentie.

Minimale informatie voor publicatie van kwantitatieve real-time PCR-experimenten (MIQE)
Het wordt gebruikt voor kwantitatieve real-time PCR en het kan ook worden gebruikt voor reverse transcriptie en het duidt gewoonlijk op reverse transcriptie-polymerase-kettingreactie en het is geen real-time PCR.

 

Aligner 25-36

KS072012-III 1 Kit
EUR 412
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

RapidSeq High Yield Directional mRNA Sample Prep Kit - With Aligner 25-36

KS073012-III 1 Kit
EUR 1142
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

RapidSeq High Yield Small RNA Sample Prep Kit - With Aligner 25-36

KS074012-III 1 Kit
EUR 1021
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Advanced Medium

C0003-04 RT 500 mL Bottle
EUR 103

Medium 199

C0012-01 RT 500 mL Bottle
EUR 105

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C03-127-10kg 10 kg
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COLIFORM MEDIUM

C03-127-2kg 2kg
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C03-127-500g 500 g
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E05-100-10kg 10 kg
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EC MEDIUM

E05-100-2Kg 2 Kg
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EC MEDIUM

E05-100-500g 500 g
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H08-107-2Kg 2 Kg
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HLP MEDIUM

H08-107-500g 500 g
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VALI Medium

450 500 ml
EUR 265

Mounting Medium

4300 3 ml
EUR 180.5
Description: This is Mounting medium (non-fading) used for maintaining optimal conditions needed to obtain the maximum fluorescence emission from Fluorescein.

BEGM Medium

451 500 ml
EUR 152

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D04-117-10kg 10 kg
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DC MEDIUM

D04-117-2kg 2kg
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DC MEDIUM

D04-117-500g 500 g
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Heller's Medium

CP014-010 10X1L
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Heller's Medium

CP014-500 50L
EUR 126

Hoagland's Medium

CP015-010 10X1L
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Hoagland's Medium

CP015-500 50L
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NM42400 125 ml
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S19-110-10kg 10 kg
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S19-110-2kg 2kg
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SD7024 250g
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SD7025 250g
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SD7031 250g
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SD7032 250g
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SD7033 250g
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DJ1018 100g
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Insect Cell Medium: TNM-FH Insect Culture Medium

ABP-MED-10001 1 liter Ask for price
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ABP-MED-10002 1 liter Ask for price
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Aqueous Mounting Medium

AR1018 10mL X5 (Enough for 800-1200 assays)
EUR 80

Antifade Mounting Medium

AR1109 10mL (for 1000 assays)
EUR 65

AddexBio Cryopreservation Medium

C0002-20mL RT vial
EUR 104

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C03-131-10kg 10 kg
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C03-131-2Kg 2 Kg
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E05-108-10kg 10 kg
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EC MEDIUM, MODIFIED

E05-108-2Kg 2 Kg
EUR 226

EC MEDIUM, MODIFIED

E05-108-500g 500 g
EUR 98

Hepatocyte Maintenance Medium

HM42600 100 ml
EUR 409

Neuron Growth Medium

HNM001 500 ml
EUR 331

Agarose, medium EEO

GE4750-100G 100 g
EUR 166

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GE4750-500G 500 g
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FLUID THIOGLYCOLLATE MEDIUM

F06-101-10kg 10 kg
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F06-101-2kg 2kg
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F06-101-500g 500 g
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FluoreGuard Mounting Medium

FMM030 30 ml
EUR 96

FluoreGuard Mounting Medium

FMM060 60 ml
EUR 116

FluoreGuard Mounting Medium

FMM500 500 ml
EUR 327

FluoreGuard Mounting Medium

FMM999 1000 ml
EUR 530

RPMI 1640 Medium

abx098879-500ml 500 ml
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A01-116-10kg 10 kg
EUR 1034

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A01-116-2Kg 2 Kg
EUR 264

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A01-116-500g 500 g
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SuperMOUNT? Mounting Medium

1211-20
EUR 201

Recovery Medium - 6x12mL

1711 4/PK
EUR 128

EverBrite Mounting Medium

23001 10mL
EUR 178
Description: Minimum order quantity: 1 unit of 10mL

MCDB 131 Medium

CM034-050 500ml
EUR 85

MCDB 131 Medium

CM034-300 6x500ml
EUR 165

MCDB 131 Medium

CM034-310 10x500ml
EUR 219

MCDB 131 Medium

CM034-320 20x500ml
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CM034-350 50x500ml
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Williams' Medium E

CM063-050 500ml
EUR 91

Williams' Medium E

CM063-300 6x500ml
EUR 266

Williams' Medium E

CM063-310 10x500ml
EUR 313

Williams' Medium E

CM063-320 20x500ml
EUR 604

Williams' Medium E

CM063-350 50x500ml
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Cereal Crops Medium

CP003-005 5L
EUR 113

Cereal Crops Medium

CP003-010 10X1L
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Kao Michayluk Medium

CP017-010 10X1L
EUR 99

Kao Michayluk Medium

CP017-500 50L
EUR 126

Orchid Maintenance Medium

CP037-010 10X1L
EUR 109

Orchid Maintenance Medium

CP037-500 50L
EUR 138

Woody Plants Medium

CP040-010 10X1L
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Woody Plants Medium

CP040-500 50L
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OF BASAL MEDIUM

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M13-121-2Kg 2 Kg
EUR 371

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M13-121-500g 500 g
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Sepadextran-25 Medium

SD25M-100 100 g
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Sepadextran-25 Medium

SD25M-500 500 g
EUR 1036

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SD50M-100 100 g
EUR 344

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SD50M-500 500 g
EUR 1285

REINFORCED CLOSTRIDIUM MEDIUM

R18-105-10kg 10 kg
EUR 1194

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R18-105-2kg 2kg
EUR 299

REINFORCED CLOSTRIDIUM MEDIUM

R18-105-500g 500 g
EUR 118

Schwann Growth Medium

SGM001 500 ml
EUR 244

TERRIFIC BROTH MEDIUM

T20-114-10kg 10 kg
EUR 675

TERRIFIC BROTH MEDIUM

T20-114-2kg 2kg
EUR 186

TERRIFIC BROTH MEDIUM

T20-114-500g 500 g
EUR 87

THIOGLYCOLLATE MEDIUM, BREWER

T20-118-10kg 10 kg
EUR 1364

THIOGLYCOLLATE MEDIUM, BREWER

T20-118-2kg 2kg
EUR 336

THIOGLYCOLLATE MEDIUM, BREWER

T20-118-500g 500 g
EUR 128

2XYT MICROBIAL MEDIUM

X24-102-10kg 10 kg
EUR 614

2XYT MICROBIAL MEDIUM

X24-102-2Kg 2 Kg
EUR 173

2XYT MICROBIAL MEDIUM

X24-102-500g 500 g
EUR 83

H Medium Broth

DJ1019 100g
EUR 60.88
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