Bacteriële celculturen in een lab-on-a-disc: een eenvoudig en veelzijdig

Bacteriële celculturen in een lab-on-a-disc: een eenvoudig en veelzijdig apparaat voor het kwantificeren van de werkzaamheid van antibiotica

Pathogene bacteriële biofilms zullen levensbedreigend zijn, de hoge kwaliteit van leven van de getroffen persoon aanzienlijk verlagen en een aanzienlijke belasting voor de gezondheidszorg vormen. Huidige strategieën voor analyse van antibacteriële therapieën in klinieken en in vitro methoden die worden gebruikt bij de groei en screening van geneesmiddelen, vergemakkelijken beide de vorming van biofilm niet of zijn lastig te functioneren, willen enorme reagensvolumes en zijn duur, waardoor hun bruikbaarheid beperkt is.

Om met deze punten om te gaan, presenteert dit werk het evenement van een stevig in vitro celtraditieplatform dat geschikt is voor confocale microscopie.

Het platform dat is gevormd als een compact disc, faciliteert een lange bacteriële traditie zonder externe pompen en slangen en zal een aantal dagen worden gebruikt zonder extra vloeistof. Pseudomonas aeruginosa biofilm wordt bijvoorbeeld gekweekt uit afzonderlijke cellen en het is bewezen dat:

1) het platform levert reproduceerbare en betrouwbare resultaten; 2) vooruitgang is afhankelijk van bewegingslading en de samenstelling van het voortgangsmedium; en drie) de doeltreffendheid van het antibioticum zal afhangen van de gevormde biofilm. Dit platform maakt de voortgang, kwantificering en remedie van biofilm mogelijk zoals in een traditionele bewegingsopstelling, terwijl de apparaatbarrière van lab-on-chip-methoden wordt verkleind. Het biedt een gebruiksvriendelijke, redelijk geprijsde mogelijkheid voor eindklanten die werken met celkweek in relatie tot b.v. diagnostiek en drugsscreening.

Bead Sample Pack - 5M Set
BSP-5Y	Next Advance	1pack	EUR 289.2
Description: Bead sample pack for Bullet Blender 5mL Storm models. Includes 10mL of: ZROB015, ZROB05, ZROB20, SSB02, SSB14B, SSB32, SSB60, SSB110 and SSUFO35.

Bead Sample Pack - 50mL Set
BSP-50B2	Next Advance	1pack	EUR 484.8
Description: Bead sample pack for Bullet Blender 50mL tube models. Includes 20mL of: ZROB05, ZROB20, SSB02, SSB14B, SSB48, SSB60, SSUFO35 and SSUFO56.

Bead Sample Pack - Full Set
BSP-ALL2	Next Advance	1pack	EUR 460.8
Description: Bead sample pack inlcudes sampling of all 20 bead types in 10mL tubes.

Bead Sample Pack - Organ Tissues
BSP-OT3	Next Advance	1pack	EUR 282
Description: Bead sample pack for homogenization of organ tissues. Includes 10mL of: ZROB05, ZROB10, ZROB20, SSB14B, SSB32 and SSUFO35.

Bead Sample Pack - Microcentrifuge Set
BSP-MC2	Next Advance	1pack	EUR 278.4
Description: Bead sample pack for Bullet Blender 1.5mL tube models. Includes 10mL of: ZROB015, ZROB05, ZROB10, ZROB20, SSB02, SSB14B, SSB32 and SSUFO35.

Water, 0.1um Filtered, cell cultures tested
W0900-050	GenDepot	500 ml	EUR 81.6

Water, 0.1um Filtered, cell cultures tested
W0900-310	GenDepot	10x500 ml	EUR 202.8

Water, 0.1um Filtered, cell cultures tested
W0900-350	GenDepot	50x500 ml	EUR 627.6

Red Blood Cell Sample Pack
88R-1050	Fitzgerald	6 x 2 ml	EUR 483.6
Description: Horse, Mouse, Rabbit, Sheep, Bovine and Chicken glutaraldehyde stabilized blood cell samples

CULTURE VESSEL, C1770 SAMPLE
C2099	PhytoTechnology Laboratories	10EA	EUR 10.56

All Sample Diluents Sample Pack
KF17355	Neuromics	3X100 ml	EUR 326.4

PTcon SAMPLE PACK
C2125	PhytoTechnology Laboratories	5EA	EUR 32.34

Growth Supplement for Anaerobic Cultures
FD805-100G	EWC Diagnostics	1 unit	EUR 16.61
Description: Growth Supplement for Anaerobic Cultures

GELLING AGENT SAMPLE PACK
G3500	PhytoTechnology Laboratories	1EA	EUR 17.71

96 Well Cell Culture Plate, White, Flat bottom, TC, Sterile, 1/pack, 100/cs
701301	NEST Biotechnology	100 pcs/cs	EUR 103.73
Description: 96 Well Cell Culture Plate, White, Flat bottom, TC, Sterile, 1/pack, 100/cs

Cell Culture Flask,3 Layer, Vent Cap, TC, Sterile,Bag Package, 1 pc/pack, 12 pak
731302	NEST Biotechnology	12 pcs/cs	EUR 65.89
Description: Cell Culture Flask,3 Layer, Vent Cap, TC, Sterile,Bag Package, 1 pc/pack, 12 pakcs/case, 12 pcs/case

Cell Culture Flask,3 Layer, Plug Seal Cap, TC, Sterile,Bag Package, 1 pc/pack, 1
731301	NEST Biotechnology	12 pcs/cs	EUR 64.9
Description: Cell Culture Flask,3 Layer, Plug Seal Cap, TC, Sterile,Bag Package, 1 pc/pack, 12 pakcs/case, 12 pcs/case

3 Blocking Buffer Sample Pack
KF17340	Neuromics	3 x 100 ml	EUR 246

All Assay Diluents Sample Pack
KF17351	Neuromics	4X100 ml	EUR 326.4

TMB Peroxidase Substrate SAMPLE PACK
42R-TB101	Fitzgerald	3 x 100 ml	EUR 393
Description: 100mL of 3 different TMB Peroxidase Substrates ready to use in immunoassays: Ultra sensitivite, Highly Sensitive, and Slow Acting.

GC/HPLC Demo Sample Pack - EACH
TNDPSSSV	Scientific Laboratory Supplies	EACH	EUR 5.4

S-adenosylmethionine ELISA kit for cell culture supernatant or other samples
IK00204	Arthus	96 tests	EUR 699
Description: S-adenosylmethionine ELISA kit for cell culture supernatant or other samples

GC Headspace Demo Sample Pack - EACH
TNDPSSHS	Scientific Laboratory Supplies	EACH	EUR 5.4

96 Well Cell Culture Plate, White, Flat bottom, Non-Treated, Sterile, 1/pack, 10
701311	NEST Biotechnology	100 pcs/cs	EUR 101.75
Description: 96 Well Cell Culture Plate, White, Flat bottom, Non-Treated, Sterile, 1/pack, 100/cs

S-adenosylhomocysteine ELISA kit for cell culture supernatant or other samples
IK00304	Arthus	96 tests	EUR 1049
Description: S-adenosylhomocysteine ELISA kit for cell culture supernatant or other samples

Anti Human Plasminogen MAb Sample Pack
MBS480366-1Pack	MyBiosource	1Pack	EUR 825

Anti Human Plasminogen MAb Sample Pack
MBS480366-5x1Pack	MyBiosource	5x1Pack	EUR 3490

CORNING® BIOCOAT™ POLY-D-LYSINE/LAMININ 8 WELL CULTURESLIDE, 4/PACK, 12/CASE
354688	CORNING	3/pk	EUR 543.6
Description: Advanced Cells - DL; Slips/Slides

MEM Eagle Modified w/o L-Glutamine (Suspension Cultures) (Powder)
MBS653395-25L	MyBiosource	25L	EUR 710

MEM Eagle Modified w/o L-Glutamine (Suspension Cultures) (Powder)
MBS653395-5x25L	MyBiosource	5x25L	EUR 2800

Human hepatocytes in monolayer-long term cultures, in 6 well-plate
LOHUF06-EU	Westburg	each	Ask for price

Human hepatocytes in monolayer-long term cultures, in 12 well-plate
LOHUF12-EU	Westburg	each	Ask for price

Human hepatocytes in monolayer-long term cultures, in 24 well-plate
LOHUF24-EU	Westburg	each	Ask for price

Human hepatocytes in monolayer-long term cultures, in 48 well-plate
LOHUF48-EU	Westburg	each	Ask for price

Human hepatocytes in monolayer-long term cultures, in 96 well-plate
LOHUF96-EU	Westburg	each	Ask for price

4% Magnetic T1 Agarose Bead
4MGT1-100	Agarose Bead Technologies	100 ml	EUR 803.25

4% Magnetic T1 Agarose Bead
4MGT1-25	Agarose Bead Technologies	25 ml	EUR 226.28

Supreme Plant Tissue Culture Grade Agar
PCTA100	Plant Cell Technology	500g	EUR 144
Description: A polysaccharide complex that is produced from the red alga Rhodophyceae. The extraction of agarocytes is obtained by bleaching and hot water. Supreme Plant Tissue Culture Grade Agar offers greater clarity of plant culture media.

Supreme Plant Tissue Culture Grade Agar
PCTA500	Plant Cell Technology	1000g	EUR 180
Description: A polysaccharide complex that is produced from the red alga Rhodophyceae. The extraction of agarocytes is obtained by bleaching and hot water. Supreme Plant Tissue Culture Grade Agar offers greater clarity of plant culture media.

4% Magnetic Large Agarose Bead
4MGT3-100	Agarose Bead Technologies	100 ml	EUR 803.25

4% Magnetic Large Agarose Bead
4MGT3-25	Agarose Bead Technologies	25 ml	EUR 226.28

Multifuge X4 Pro cell culture package - EACH
75016067	Scientific Laboratory Supplies	EACH	EUR 18158.85

Multifuge X4R Pro cell culture package - EACH
75016066	Scientific Laboratory Supplies	EACH	EUR 24460.65

Nickel NTA Magnetic Agarose Bead (5%)
MagNTANi5-10	Agarose Bead Technologies	10ml suspension	EUR 327.63

Nickel NTA Magnetic Agarose Bead (5%)
MagNTANi5-2	Agarose Bead Technologies	2ml suspension	EUR 79.33

Nickel NTA Magnetic Agarose Bead (5%)
MagNTANi5-5	Agarose Bead Technologies	5ml suspension	EUR 180.71

Recombinant Arabidopsis thaliana Auxin-induced in root cultures protein 12 (AIR12)
MBS1330787-002mgBaculovirus	MyBiosource	0.02mg(Baculovirus)	EUR 1125

Recombinant Arabidopsis thaliana Auxin-induced in root cultures protein 12 (AIR12)
MBS1330787-002mgEColi	MyBiosource	0.02mg(E-Coli)	EUR 705

Recombinant Arabidopsis thaliana Auxin-induced in root cultures protein 12 (AIR12)
MBS1330787-002mgYeast	MyBiosource	0.02mg(Yeast)	EUR 875

Recombinant Arabidopsis thaliana Auxin-induced in root cultures protein 12 (AIR12)
MBS1330787-01mgEColi	MyBiosource	0.1mg(E-Coli)	EUR 845

Recombinant Arabidopsis thaliana Auxin-induced in root cultures protein 12 (AIR12)
MBS1330787-01mgYeast	MyBiosource	0.1mg(Yeast)	EUR 1025

Sample Diluent Optimization Pack: 100mL SD1, SD2, SD3, PFSD
959	Immunochemistry	pack	EUR 187

Trueprep® AUTO MTB Sample Pre-treatment Pack
60204AS05	Molbio Diagnostics Private Limited	5T	EUR 5

Trueprep® AUTO MTB Sample Pre-treatment Pack
60204AS20	Molbio Diagnostics Private Limited	20T	EUR 20

Trueprep® AUTO MTB Sample Pre-treatment Pack
60205AS50	Molbio Diagnostics Private Limited	50T	EUR 50

Sample Diluent Optimization Pack: All Three 100mL SDs
MBS258064-1Pack	MyBiosource	1Pack	EUR 265

Sample Diluent Optimization Pack: All Three 100mL SDs
MBS258064-5Packs	MyBiosource	5Packs	EUR 1010

Human Fibronectin Coated Culture Ware - T25 10flasks/pack
cAP-5001-t25	Angio Proteomie	1Pack	EUR 198

Human Fibronectin Coated Culture Ware - T75 10flasks/pack
cAP-5001-t75	Angio Proteomie	1Pack	EUR 316.8

Human Collagen I Coated Culture Ware - T25 10flasks / pack
cAP-5002-t25	Angio Proteomie	1Pack	EUR 198

Human Collagen I Coated Culture Ware - T75 10flasks / pack
cAP-5002-t75	Angio Proteomie	1Pack	EUR 316.8

SL4R Plus Centrifuge Cell Culture TX1000 Package - EACH
75016075	Scientific Laboratory Supplies	EACH	EUR 20652.3

Trueprep® AUTO Universal Sample Pre-treatment Pack
60205AB05	Molbio Diagnostics Private Limited	5T	EUR 5

Trueprep® AUTO Universal Sample Pre-treatment Pack
60205AB20	Molbio Diagnostics Private Limited	20T	EUR 20

Trueprep® AUTO Universal Sample Pre-treatment Pack
60205AB50	Molbio Diagnostics Private Limited	50T	EUR 50

QRRPT1 Rapid Run™ (High Sub) Agarose Bead
QRRPT1-25	Agarose Bead Technologies	25 ml	EUR 72.04

QRRPT1 Rapid Run™ (High Sub) Agarose Bead
QRRPT1-300	Agarose Bead Technologies	300 ml	EUR 497.73

Human Collagen I Coated Culture Ware - 12 wells 5plates/ pack
cAP-5002-w12	Angio Proteomie	1Pack	EUR 168.3

Human Collagen I Coated Culture Ware - 24 wells 5plates/ pack
cAP-5002-w24	Angio Proteomie	1Pack	EUR 178.2

Human Collagen I Coated Culture Ware - 384 wells 5plates/ pack
cAP-5002-w384	Angio Proteomie	1Pack	EUR 396

Human Collagen I Coated Culture Ware - 48 wells 5plates/ pack
cAP-5002-w48	Angio Proteomie	1Pack	EUR 198

Human Collagen I Coated Culture Ware - 6 wells 5plates/ pack
cAP-5002-w6	Angio Proteomie	1Pack	EUR 158.4

Human Collagen I Coated Culture Ware - 96 wells 5plates/ pack
cAP-5002-w96	Angio Proteomie	1Pack	EUR 247.5

Human Fibronectin Coated Culture Ware - 12 wells 5 plates/pack
cAP-5001-w12	Angio Proteomie	1Pack	EUR 163.35

Human Fibronectin Coated Culture Ware - 24 wells 5 plates/pack
cAP-5001-w24	Angio Proteomie	1Pack	EUR 178.2

Human Fibronectin Coated Culture Ware - 384 wells 5 plates/pack
cAP-5001-w384	Angio Proteomie	1Pack	EUR 396

Human Fibronectin Coated Culture Ware - 48 wells 5 plates/pack
cAP-5001-w48	Angio Proteomie	1Pack	EUR 198

Human Fibronectin Coated Culture Ware - 6 wells 5 plates/pack
cAP-5001-w6	Angio Proteomie	1Pack	EUR 158.4

Human Fibronectin Coated Culture Ware - 96 wells 5 plates/pack
cAP-5001-w96	Angio Proteomie	1Pack	EUR 247.5

Corning 100mm TC-Treated Culture Dishes in 6-pack Carriers - PK480
430293	Scientific Laboratory Supplies	PK480	EUR 571.05

Total RNA Mini Kit (Blood/Cultured Cell) - Individual Packed Columns
RB004	Geneaid Biotech	each	EUR 3

Total RNA Mini Kit (Blood/Cultured Cell) - Individual Packed Columns
RB050	Geneaid Biotech	each	EUR 82.3

Total RNA Mini Kit (Blood/Cultured Cell) - Individual Packed Columns
RB100	Geneaid Biotech	each	EUR 150.7

Total RNA Mini Kit (Blood/Cultured Cell) - Individual Packed Columns
RB300	Geneaid Biotech	each	EUR 396.5

Gryphon Ecotropic Packaging Cell Line for Retrovirus (1 x 106 cells, Culture)
ABP-RVC-10002C	Allele Biotech	1 flask	Ask for price
Description: PRODUCT LINE DISCONTINUED. Please contact us.

CORNING® BIOCOAT™ POLY-D-LYSINE 60MM CULTURE DISHES, 20/PACK, 100/CASE, NONSTERILE
356468	CORNING	20/pk	EUR 469.2
Description: Advanced Cells - DL; Dishes BCT

Gryphon Amphotropic Packaging Cell Line for Retrovirus (1 x 106 cells, Culture)
ABP-RVC-10001C	Allele Biotech	1 flask	Ask for price
Description: PRODUCT LINE DISCONTINUED. Please contact us.

CORNING® BIOCOAT™ COLLAGEN I 35MM TC-TREATED CULTURE DISHES, 100/PACK, 100/CASE, NONSTERILE
356456	CORNING	20/pk	EUR 469.2
Description: Advanced Cells - DL; Dishes BCT

Total RNA Mini Kit (Blood/Cultured Cell) w/DNase Set - Individual Packed Columns
RBD004	Geneaid Biotech	each	EUR 5

Total RNA Mini Kit (Blood/Cultured Cell) w/DNase Set - Individual Packed Columns
RBD050	Geneaid Biotech	each	EUR 102.3

Total RNA Mini Kit (Blood/Cultured Cell) w/DNase Set - Individual Packed Columns
RBD100	Geneaid Biotech	each	EUR 190.7

Total RNA Mini Kit (Blood/Cultured Cell) w/DNase Set - Individual Packed Columns
RBD300	Geneaid Biotech	each	EUR 506.5

Cell Culture Grade Water, Cell Culture Tested, Sterile
CCM1081-500	Bio Basic	500 mL	EUR 73.49

Cell Culture Grade Water, Cell Culture Tested, Sterile
CCM2081-1000	Bio Basic	1000 mL	EUR 82.64

Protein A Tell-Tale Gold® Ribbon Sample Pack, 3 x (1 cm x 30 cm)
TTGA-SP3-B090	BioAssayWorks	3 x (1 cm x 30 cm)	EUR 208.7

Protein G Tell-Tale Gold® Ribbon Sample Pack, 3 x (1 cm x 30 cm)
TTGG-SP3-B090	BioAssayWorks	3 x (1 cm x 30 cm)	EUR 208.7

ExoQualiTM Human Exosome Capture and Isolation Kits (for Cell Culture Media/ Urine) (CD9 Capture Beads)
CEIK-097L-10T	Creative Bioarray	10T	Ask for price

ExoQualiTM Human Exosome Capture and Isolation Kits (for Cell Culture Media/ Urine) (CD63 Capture Beads)
CEIK-098L-10T	Creative Bioarray	10T	Ask for price

ExoQualiTM Human Exosome Capture and Isolation Kits (for Cell Culture Media/ Urine) (CD81 Capture Beads)
CEIK-099L-10T	Creative Bioarray	10T	Ask for price

ExoQualiTM Mouse Exosome Capture and Isolation Kits (for Cell Culture Media/ Urine) (mCD9 Capture Beads)
CEIK-104L-10T	Creative Bioarray	10T	Ask for price

CORNING® BIOCOAT™ POLY-D-LYSINE 35MM TC-TREATED CULTURE DISHES, 20/PACK, 100/CASE, NONSTERILE
356467	CORNING	20/pk	EUR 468
Description: Advanced Cells - DL; Dishes BCT

CORNING® BIOCOAT™ POLY-L-LYSINE 60MM TC-TREATED CULTURE DISHES, 20/PACK, 100/CASE, NONSTERILE
356517	CORNING	20/pk	EUR 481.2
Description: Advanced Cells - DL; Dishes BCT

CORNING® BIOCOAT™ POLY-L-LYSINE 35MM TC-TREATED CULTURE DISHES, 20/PACK, 100/CASE, NONSTERILE
356518	CORNING	20/pk	EUR 481.2
Description: Advanced Cells - DL; Dishes BCT

ExoQualiTM Mouse Exosome Capture and Isolation Kits (for Cell Culture Media/ Urine) (mCD63 Capture Beads)
CEIK-105L-10T	Creative Bioarray	10T	Ask for price

ExoQualiTM Mouse Exosome Capture and Isolation Kits (for Cell Culture Media/ Urine) (mCD9/CD63 Capture Beads)
CEIK-106L-10T	Creative Bioarray	10T	Ask for price

Protein A/G Tell-Tale Gold® Ribbon Sample Pack, 3 x (1 cm x 30 cm)
TTGAG-SP3-B090	BioAssayWorks	3 x (1 cm x 30 cm)	EUR 208.7

ExoQualiTM Human Exosome Capture and Isolation Kits (for Cell Culture Media/ Urine) (CD9/CD63/CD81 Capture Beads)
CEIK-100L-10T	Creative Bioarray	10T	Ask for price

CORNING® BIOCOAT™ LAMININ 35MM TC-TREATED CULTURE DISHES, 5/PACK, 20/CASE, NONSTERILE
354458	CORNING	20/pk	EUR 267.6
Description: Advanced Cells - DL; Dishes BCT

CORNING® BIOCOAT™ GELATIN 100MM TC-TREATED CULTURE DISHES, 10/PACK, 40/CASE, NONSTERILE
356653	CORNING	10/pk	EUR 262.8
Description: Advanced Cells - DL; Dishes BCT

Platinum-E Retroviral Packaging Cell Line, Ecotropic
RV-101	Cell Biolabs	1 vial	EUR 770

Platinum-GP Retroviral Packaging Cell Line, Pantropic
RV-103	Cell Biolabs	1 vial	EUR 770

Platinum-A Retroviral Packaging Cell Line, Amphotropic
RV-102	Cell Biolabs	1 vial	EUR 770

Cell Culture Plate
CCP01000-00-CellCulturePlate6Well2CellCulturePlate96Well2	Vazyme	Cell Culture Plate，6 Well*2Cell Culture Plate，96 Well*2	EUR 1.09

CORNING® BIOCOAT™ MATRIGEL® MATRIX THIN LAYER 100MM CULTURE DISHES, 10/PACK, 10/CASE, NONSTERILE
354600	CORNING	5/pk	EUR 291.6
Description: Advanced Cells - DL; Dishes BCT

CORNING® BIOCOAT™ MATRIGEL® MATRIX THIN LAYER 60MM CULTURE DISHES, 5/PACK, 20/CASE, NONSTERILE
354601	CORNING	5/pk	EUR 330
Description: Advanced Cells - DL; Dishes BCT

CORNING® BIOCOAT™ MATRIGEL® MATRIX THIN LAYER 35MM CULTURE DISHES, 20/PACK, 20/CASE, NONSTERILE
354602	CORNING	10/pk	EUR 304.8
Description: Advanced Cells - DL; Dishes BCT

CORNING® BIOCOAT™ POLY-D-LYSINE 100MM TC-TREATED CULTURE DISHES, 10/PACK, 40/CASE, NONSTERILE
356469	CORNING	10/pk	EUR 262.8
Description: Advanced Cells - DL; Dishes BCT

MDR Sampler Pack antibody
STJ16100969	St John's Laboratory	1 pack	EUR 799.2

Water for Cell Culture
TBS5050	Tribioscience	500ml	EUR 10

Magnetic COOH Sampler Pack
BLI21940-1	Polysciences Europe GmbH	5ml	EUR 395

T225 Cell Culture Flask
P0230	ABM	25/case	EUR 185

Cell Culture Grade Water
25-511	Genesee Scientific	6 x 500 mL/Unit	EUR 52.91
Description: Cell Culture Tested, Sterile

Cell Culture Grade Water
25-511B	Genesee Scientific	6 x 1000 mL	EUR 85.86
Description: Cell Culture Tested, Sterile

Water, Cell Culture Grade
CA018-600	GenDepot	6x1000ml	EUR 148.8

Hepes Cell Culture Grade
AK3268-0250	Akron Biotech	250g	Ask for price

Hepes Cell Culture Grade
AK3268-0500	Akron Biotech	500g	Ask for price

Hepes Cell Culture Grade
AK3268-1000	Akron Biotech	1kg	Ask for price

CELL CULTURE GRADE WATER
IB42010	IBI Scientific	1L	EUR 41.13
Description: DBL DIST. - 0.1 MICRON STERILE

CELL CULTURE GRADE WATER
IB42011	IBI Scientific	6x1L	EUR 239.38
Description: DBL DIST. - 0.1 MICRON STERILE

CELL CULTURE GRADE WATER
IB42012	IBI Scientific	12x1L	EUR 469.02
Description: DBL DIST. - 0.1 MICRON STERILE

CELL CULTURE GRADE WATER
IB42020	IBI Scientific	2L	EUR 59.67
Description: DBL DIST. - 0.1 MICRON STERILE

CELL CULTURE GRADE WATER
IB42021	IBI Scientific	6x2L	EUR 342.87
Description: DBL DIST. - 0.1 MICRON STERILE

CELL CULTURE GRADE WATER
IB42030	IBI Scientific	10L	EUR 194.72
Description: JUG W/HANDL 0.1 MICRON STERILE

25 cm2 Cell Culture Flask
CCF00025-00-10Bag	Vazyme	10 / Bag	EUR 1.09

25 cm2 Cell Culture Flask
CCF00025-200Case	Vazyme	200 / Case	EUR 186.39

75 cm2 Cell Culture Flask
CCF00075-00-5Bag	Vazyme	5 / Bag	EUR 1.09

75 cm2 Cell Culture Flask
CCF00075-100Case	Vazyme	100 / Case	EUR 176.04

Human MEP Cell Culture Medium (human megakaryocyte–erythroid progenitor cell culture medium)
P770	101Bio	8 rxn	EUR 499

ROCKER CELL CULTURE BAG, 2L
91-200-80	CORNING	1/pk	EUR 450
Description: Flexible Containers; Bioprocess Bags and Parts

175 cm2 Cell Culture Flask
CCF00175-40Case	Vazyme	40 / Case	EUR 119.36

225 cm2 Cell Culture Flask
CCF00225-25Case	Vazyme	25 / Case	EUR 93.2

Cell Culture Plate, 6 Well
CCP01006-50Case	Vazyme	50 / Case	EUR 61.59

Cell Culture Plate, 96 Well
CCP01096-100Case	Vazyme	100 / Case	EUR 154.24

Sample Cell 10ml Matched - PK2
2495402	Scientific Laboratory Supplies	PK2	EUR 460.35

ROCKER CELL CULTURE BAG, 20L
91-200-78	CORNING	1/pk	EUR 493.2
Description: Flexible Containers; Bioprocess Bags and Parts

ROCKER CELL CULTURE BAG, 10L
91-200-79	CORNING	1/pk	EUR 446.4
Description: Flexible Containers; Bioprocess Bags and Parts

ROCKER CELL CULTURE BAG, 50L
91-200-81	CORNING	1/pk	EUR 570
Description: Flexible Containers; Bioprocess Bags and Parts

Mouse ES Cell Culture Media
M16101	Neuromics	500 mL	EUR 1336.8

CRFK CELL TUBE CULTURE
63-0600	Quidel	each	Ask for price

6-well Cell Culture Plates
abx090688-50plates	Abbexa	50 plates	EUR 571.2

6-well Cell Culture Plates
abx090688-6plates	Abbexa	6 plates	EUR 142.8

6-Well Cell Culture Plates
25-105	Genesee Scientific	100 Plates/Unit	EUR 119.75
Description: Flat Bottom Wells, TC Treated

HEPES. cell culture tested - kg
PM-B2093/1000	Biosera France - Medical Scientific Equipment	kg	EUR 146.69

6-well Cell Culture Plates
abx090688-100l	Abbexa	100 µl	EUR 125

6-well Cell Culture Plates
abx090688-1ml	Abbexa	1 ml	Ask for price

6-well Cell Culture Plates
abx090688-200l	Abbexa	200 µl	EUR 306.25

12-Well Cell Culture Plates
25-106	Genesee Scientific	100 Plates/Unit	EUR 123.27
Description: Flat Bottom Wells, TC Treated

24-Well Cell Culture Plates
25-107	Genesee Scientific	100 Plates/Unit	EUR 125.99
Description: Flat Bottom Wells, TC Treated

48-Well Cell Culture Plates
25-108	Genesee Scientific	100 Plates/Unit	EUR 138.07
Description: Flat Bottom Wells, TC Treated

96-Well Cell Culture Plates
25-109	Genesee Scientific	100 plates	EUR 117.72
Description: Flat Bottom Wells, TC Treated

96-Well Cell Culture Plates
25-221	Genesee Scientific	100 plates	EUR 139.75
Description: U-Bottom Wells, TC Treated

Cell Culture Inserts, 6-Well
25-281	Genesee Scientific	6 Inserts/Plate, 24/Unit	EUR 71.41
Description: 1µm PC Membrane, Sterilized

Cell Culture Inserts, 6-Well
25-282	Genesee Scientific	6 Inserts/Plate, 24/Unit	EUR 71.41
Description: 3µm PC Membrane, Sterilized

Cell Culture Inserts, 6-Well
25-283	Genesee Scientific	6 Inserts/Plate, 24/Unit	EUR 71.41
Description: 8µm PC Membrane, Sterilized

Imiquimod for cell culture, 98%
75054	Sisco Laboratories	250 Mg	EUR 133.22
Description: Part B1

Fish Serum (cell culture grade)
GWB-AE89C9	GenWay Biotech	10 ml	Ask for price

HEPES. cell culture tested - 100g
PM-B2093/100	Biosera France - Medical Scientific Equipment	100g	EUR 23.21

HEPES. cell culture tested - 250g
PM-B2093/250	Biosera France - Medical Scientific Equipment	250g	EUR 44

Doorzichtig papier van microkristallijne cellulose / polyvinylalcohol als nieuw platform voor 3D-celcultuur

Meerlagig en gestapeld cellulosepapier is naar voren gekomen als een veelbelovend platform voor het bouwen van een driedimensionale (3D) celtraditie, dankzij de lage prijs, goede biocompatibiliteit en overmatige porositeit. Desalniettemin maakt de slechte, milde transmissie het moeilijk om celgedrag (bijv. Voortgang en proliferatie) onmiddellijk en duidelijk te volgen op het op papier gebaseerde platform met behulp van een optische microscoop.

Voor dit werk hebben we een helder microkristallijn cellulose / polyvinylalcohol (MCC / PVA) papier ontwikkeld met onregelmatige poriën via oplossen en regeneratie van microkristallijne nanocellulose (MCC), toevoeging van porogeen reagens (NaCl) en vervolgens onderdompelen in PVA-opties. Het heldere MCC-papier vertoont overmatige porositeit (maar liefst 90%), instelbare poriënmeting (tussen 23 μm en 46 μm) en enorme dikte (van 315 μm tot 436 μm) en overmatige milde transmissie onder water (> 95%).

• Via een aanvullende modificatie van helder MCC-papier met PVA, vertoont het verkregen heldere MCC / PVA-papier verbeterde mechanische eigenschappen (droge en vochtige sterkte), goede hydrofiliciteit (met een contacthoek van 70,8 °) en verbeterde biocompatibiliteit (levensvatbaarheid van de cellen zelfs 90 %). Door een aantal lagen van het heldere MCC / PVA-papier te stapelen en ontstapelen, is het gebruikt voor elk 2D- en 3D-celtraditieplatform.

• Het heldere MCC / PVA-papier onder water maakt elke directe verklaring van celmorfologie mogelijk met een optische microscoop door middel van blote ogen en fluorescentiemicroscoop na kleuring. We stellen ons voor dat het ontwikkelde, heldere MCC / PVA-papier een mooi potentieel biedt voor toekomstige doeleinden op tal van bioanalytische en biomedische gebieden, vergelijkbaar met het screenen van geneesmiddelen, tissue engineering en organ-on-chips.

Natuurequivalente verbindingen en natuurlijke zuren verbeteren en stoppen de schade veroorzaakt door een ontstekingsprobleem in Caco-2 celcultuur

Bioactieve verbindingen, vergelijkbaar met natuurlijke zuren (OA) en natuuridentieke verbindingen (NIC), kunnen een baan uitoefenen binnen de veiligheid van de werking van het darmslijmvlies, die toe te schrijven is aan hun organische eigenschappen.

Het doel van dit onderzoek was om de functie te begrijpen van twee OA (citroenzuur en sorbinezuur) en een paar NIC’s (thymol en vanilline), alleen of gemengd in een mix (OA + NIC), op de prestatie van de darmbarrière, beide gedurende homeostatische situatie of tijdens een ontstekingsprobleem uitgevoerd met pro-inflammatoire cytokines en lipopolysacchariden (LPS). Het onderzoek werd uitgevoerd op de menselijke epitheliale cellijn Caco-2, een bekende mannequin van de intestinale epitheliale barrière.

De resultaten bevestigden hoe OA en NIC alleen de transepitheliale elektrische weerstand (TEER) en mRNA-reeksen van tight junction (TJ) -delen kunnen verbeteren, maar OA + NIC bevestigde een sterkere werkzaamheid in vergelijking met de afzonderlijke moleculen.

Wanneer zich een ontstekingsprobleem voordeed, was OA + NIC-mix in een positie om elk letsel te verbeteren en te stoppen als gevolg van de pro-inflammatoire stimulus, waardoor de daling van TEER werd verminderd of gestopt en de TJ-mRNA-expressie werd versterkt. De informatie ondersteunt de functie van OA + NIC bij het moduleren van de darmstaaf

Realtime polymerasekettingreactie (realtime PCR)

Het is ook bekend als een kwantitatieve polymerasekettingreactie. Tijdens de PCR controleert het de toepassing van een gericht DNA. Omdat het een laboratoriumtechniek van moleculaire biologie is die is gebaseerd op de polymerasekettingreactie. Real-time PCR wordt kwantitatief op twee manieren gebruikt, d.w.z. semi-kwantitatief en als de hoeveelheid DNA lager of hoger is, zal het semi-kwantitatieve real-time PCR zijn.

Het is de methode die helpt om de hoeveelheid PR-product te bepalen. Een kwantitatieve polymerasekettingreactie wordt ook wel real-time PCR genoemd. Iedereen die de afgelopen dagen heeft geprobeerd kwantisatie uit te voeren, herinnert zich experimenten waarin nucleïnezurextracten werden uitgevoerd om tot een verdunning te komen die een bereik van de test zou zijn. Dit heeft geleid tot een ongebruikelijk algoritme met betrekking tot het falen van controles voor kwalitatieve nucleïnezuuramplificatietesten.

Methoden van PCR

• Niet-specifieke fluorescerende kleurstoffen.
Het is een kleurstof die intercaleert met elk van het dubbelstrengs DNA.
• Sequentiespecifieke DNA-sondes
Het is gelabeld met een fluorescerende reporter, die de detectie alleen mogelijk maakt na hybridisatie van de sonde met zijn complementaire sequentie.

Minimale informatie voor publicatie van kwantitatieve real-time PCR-experimenten (MIQE)
Het wordt gebruikt voor kwantitatieve real-time PCR en het kan ook worden gebruikt voor reverse transcriptie en het duidt gewoonlijk op reverse transcriptie-polymerase-kettingreactie en het is geen real-time PCR.

 

Prigrow VI Medium
TM016	ABM	500ml	EUR 145

Prigrow IX Medium
TM019	ABM	500ml	EUR 145

Prigrow XV Medium
TM027	ABM	500 ml	EUR 145

Prigrow CI Medium
TM101	ABM	500ml	EUR 145

Prigrow X.1 Medium
TM010	ABM	1 Kit	EUR 385

Prigrow XII Medium
TM012	ABM	500ml	EUR 125

Prigrow XIV Medium
TM014	ABM	500 ml	EUR 195

Prigrow VII Medium
TM017	ABM	500ml	EUR 145

Prigrow XIII Medium
TM013	ABM	500 ml	EUR 125

Prigrow VIII Medium
TM018	ABM	500ml	EUR 145

Prigrow XVIII Medium
TM039	ABM	500 ml	EUR 195

EXS III Basal Medium
30630164-1	Glycomatrix	25 L	EUR 23.69

EXS III Basal Medium
30630164-2	Glycomatrix	50 L	EUR 43

Artificial Seawater Nutrient Medium (III)
PT153-10X1L	EWC Diagnostics	1 unit	EUR 12.95
Description: Artificial Seawater Nutrient Medium (III)

Artificial Seawater Nutrient Medium (III)
PT153-25L	EWC Diagnostics	1 unit	EUR 25.14
Description: Artificial Seawater Nutrient Medium (III)

Artificial Seawater Nutrient Medium (III)
PT153-5L	EWC Diagnostics	1 unit	EUR 5.63
Description: Artificial Seawater Nutrient Medium (III)

Hosta Rooting Medium Stage III
30630167-3	Glycomatrix	25 L	EUR 61.84

Hosta Rooting Medium Stage III
30630167-4	Glycomatrix	50 L	EUR 116.82

EXS III Basal Salt Medium, w/ Adenine
30630163-1	Glycomatrix	25 L	EUR 21.41

EXS III Basal Salt Medium, w/ Adenine
30630163-2	Glycomatrix	50 L	EUR 36.33

KATO III [KATO 3] Cells Complete Medium
CM-0372-125mL4	Elabscience Biotech	125 mL×4	EUR 108
Description: Complete Growth Medium

KATO III [KATO 3] Cells Complete Medium
CM-0372	Elabscience Biotech	125mL×4	EUR 108
Description: Cell lines complete growth medium

KATO III [KATO 3] Cells Complete Medium
MBS2568801-4x125mL	MyBiosource	4x125mL	EUR 160

Pringsheim's Medium
M698-100G	EWC Diagnostics	1 unit	EUR 23.3
Description: Pringsheim's Medium

BAT Medium (Alicyclobacillus Medium)
M1561-500G	EWC Diagnostics	1 unit	EUR 43.27
Description: BAT Medium (Alicyclobacillus Medium)

Cooked M Medium (R.C. Medium)
M149-100G	EWC Diagnostics	1 unit	EUR 27.32
Description: Cooked M Medium (R.C. Medium)

Cooked M Medium (R.C. Medium)
M149-500G	EWC Diagnostics	1 unit	EUR 130.49
Description: Cooked M Medium (R.C. Medium)

MSC Prime Medium, 25x
TBS8022-01	Tribioscience	1 mL	EUR 76

MSC Prime Medium, 25x
TBS8022-05	Tribioscience	5 mL	EUR 268

MSC Prime Medium, 25x
TBS8022-25	Tribioscience	25 mL	EUR 968

Lowenstein - Jensen Medium (L.J. Medium)
MM162-100G	EWC Diagnostics	1 unit	EUR 12.1
Description: Lowenstein - Jensen Medium (L.J. Medium)

Lowenstein - Jensen Medium (L.J. Medium)
MM162-500G	EWC Diagnostics	1 unit	EUR 34.85
Description: Lowenstein - Jensen Medium (L.J. Medium)

ATCC Medium 1754: PETC Medium (Tetra Pac
M2104-500G	EWC Diagnostics	1 unit	EUR 63.72
Description: ATCC Medium 1754: PETC Medium (Tetra Pac

Human iPS Growth Medium (PSGro Medium)
TBS8023	Tribioscience	500 mL	EUR 189

Rat Cartilage Growth Medium PrimaCell: Normal Articular Cartilage Growth Medium
9-25023	CHI Scientific	5 x 100 ml	Ask for price

Medium 199
C0012-01	Addexbio	RT 500 mL Bottle	EUR 34

Medium 199
MBS2557648-10L	MyBiosource	10L	EUR 165

Medium 199
MBS2557648-50L	MyBiosource	50L	EUR 345

Medium 199
MBS2557648-5L	MyBiosource	5L	EUR 150

Medium 199
MBS2557648-5x50L	MyBiosource	5x50L	EUR 1060

Human Cartilage Growth Medium PrimaCell: Normal Articular Cartilage Growth Medium
9-46043	CHI Scientific	5 x 100 ml	Ask for price

HS Medium (Hydrosulphite of Sodium Medium)
77707	Sisco Laboratories	100 Gms	EUR 7.7
Description: Part D

HS Medium (Hydrosulphite of Sodium Medium)
77707-1	Sisco Laboratories	500 Gms	EUR 25.66
Description: Part D

SIM Medium (Sulfite Indole Motility Medium)
49810	Sisco Laboratories	100 Gms	EUR 6.84
Description: Part D

SIM Medium (Sulfite Indole Motility Medium)
49810-1	Sisco Laboratories	500 Gms	EUR 27.37
Description: Part D

Listeria Enrichment Medium Base (UVM Medium)
28685	Sisco Laboratories	100 Gms	EUR 11.97
Description: Part D

Listeria Enrichment Medium Base (UVM Medium)
28685-1	Sisco Laboratories	500 Gms	EUR 54.74
Description: Part D

MlO Medium (Motility Indole Ornithine Medium)
88736	Sisco Laboratories	100 Gms	EUR 7.7
Description: Part D

MlO Medium (Motility Indole Ornithine Medium)
88736-1	Sisco Laboratories	500 Gms	EUR 31.64
Description: Part D

Phyto Proteose Agar Medium Base (KB Medium)
PHM008-100G	EWC Diagnostics	1 unit	EUR 13.81
Description: Phyto Proteose Agar Medium Base (KB Medium)

Phyto Proteose Agar Medium Base (KB Medium)
PHM008-500G	EWC Diagnostics	1 unit	EUR 60.56
Description: Phyto Proteose Agar Medium Base (KB Medium)

MIU Medium Base (Motility Indole Urea Medium)
16309	Sisco Laboratories	100 Gms	EUR 9.41
Description: Part D

MIU Medium Base (Motility Indole Urea Medium)
16309-1	Sisco Laboratories	500 Gms	EUR 35.07
Description: Part D

EC MEDIUM
E05-100-10kg	Alphabiosciences	10 kg	EUR 976.8

EC MEDIUM
E05-100-2Kg	Alphabiosciences	2 Kg	EUR 259.2

EC MEDIUM
E05-100-500g	Alphabiosciences	500 g	EUR 114

DC MEDIUM
D04-117-10kg	Alphabiosciences	10 kg	EUR 1894.8

DC MEDIUM
D04-117-2kg	Alphabiosciences	2kg	EUR 458.4

DC MEDIUM
D04-117-500g	Alphabiosciences	500 g	EUR 168

A Medium
DJ1018	Bio Basic	100g	EUR 101.76

VP Medium
M662-500G	EWC Diagnostics	1 unit	EUR 52.25
Description: VP Medium

E.T. Medium
M854-500G	EWC Diagnostics	1 unit	EUR 36.34
Description: E.T. Medium

E.T. Medium
M854-5KG	EWC Diagnostics	1 unit	EUR 355.04
Description: E.T. Medium

HS Medium
M245-500G	EWC Diagnostics	1 unit	EUR 34.73
Description: HS Medium

X6 MEDIUM
X8458	PhytoTechnology Laboratories	10L	EUR 30.71

M9 Medium
SD7024	Bio Basic	250g	EUR 86.1

YM Medium
SD7031	Bio Basic	250g	EUR 84.54

LB Medium
TBS8056	Tribioscience	1L	EUR 36

OF Medium - 500G
MED1664	Scientific Laboratory Supplies	500G	EUR 89.1

NLN Medium
30630102-2	Glycomatrix	25 L	EUR 19.28

NLN Medium
30630102-3	Glycomatrix	50 L	EUR 39.2

TAP Medium
30639002-1	Glycomatrix	100 g	EUR 48.32

TAP Medium
30639002-2	Glycomatrix	500 g	EUR 200.66

RWC Medium
M500-100G	EWC Diagnostics	1 unit	EUR 15.93
Description: RWC Medium

RWC Medium
M500-500G	EWC Diagnostics	1 unit	EUR 43.27
Description: RWC Medium

PNY Medium
M835-100G	EWC Diagnostics	1 unit	EUR 34.17
Description: PNY Medium

PNY Medium
M835-500G	EWC Diagnostics	1 unit	EUR 103.52
Description: PNY Medium

SIM Medium
M181-100G	EWC Diagnostics	1 unit	EUR 12.27
Description: SIM Medium

SIM Medium
M181-500G	EWC Diagnostics	1 unit	EUR 35.37
Description: SIM Medium

AKI Medium
M1879-500G	EWC Diagnostics	1 unit	EUR 36.24
Description: AKI Medium

MIU Medium
LQ092-25X10ML	EWC Diagnostics	1 unit	EUR 14.98
Description: MIU Medium

MIU Medium
LQ092-50X10ML	EWC Diagnostics	1 unit	EUR 24.33
Description: MIU Medium

SIM Medium
GM181-500G	EWC Diagnostics	1 unit	EUR 40.65
Description: SIM Medium

YEP MEDIUM
Y889	PhytoTechnology Laboratories	1L	EUR 75.28

HLP MEDIUM
H08-107-10kg	Alphabiosciences	10 kg	EUR 2733.6

HLP MEDIUM
H08-107-2Kg	Alphabiosciences	2 Kg	EUR 640.8

HLP MEDIUM
H08-107-500g	Alphabiosciences	500 g	EUR 218.4

SIM MEDIUM
S19-110-10kg	Alphabiosciences	10 kg	EUR 1225.2

SIM MEDIUM
S19-110-2kg	Alphabiosciences	2kg	EUR 313.2

SIM MEDIUM
S19-110-500g	Alphabiosciences	500 g	EUR 128.4

SOB MEDIUM
S19-124-10kg	Alphabiosciences	10 kg	EUR 1158

SOB MEDIUM
S19-124-2kg	Alphabiosciences	2kg	EUR 298.8

SOB MEDIUM
S19-124-500g	Alphabiosciences	500 g	EUR 124.8

M63 Medium
SD7033	Bio Basic	250g	EUR 86.1

MSC Medium
TBS8021	Tribioscience	500 mL	EUR 245

SOB Medium
TBS8057	Tribioscience	1L	EUR 45

SOC Medium
TBS8058	Tribioscience	100 mL	EUR 35

RM-01 Refeed Medium (zero-Serum Refeed medium)
10-310100	Quidel	100 mL	Ask for price
Description: A defined medium with EMEM with EBSS, and Glutamine. Sterile.

VALI Medium
0450	AthenaES	500 ml	EUR 177.1

BEGM Medium
0451	AthenaES	500 ml	EUR 89.6

TM4G Medium
30630177-2	Glycomatrix	25 L	EUR 22.1

TM4G Medium
30630177-3	Glycomatrix	50 L	EUR 41.14

Burk's Medium
M707-500G	EWC Diagnostics	1 unit	EUR 41.14
Description: Burk's Medium

M9CA Medium
68957	Sisco Laboratories	100 Gms	EUR 21.38
Description: Part D

M9CA Medium
68957-1	Sisco Laboratories	500 Gms	EUR 86.38
Description: Part D

mD5A MEDIUM
M5516	PhytoTechnology Laboratories	10L	EUR 258.64

M9CA Medium
SD7025	Bio Basic	250g	EUR 86.1

Insect Cell Medium: TNM-FH Insect Culture Medium
ABP-MED-10001	Allele Biotech	1 liter	Ask for price

Aseptic Commissioning Medium (Aseptic Packing Line Medium)
15577	Sisco Laboratories	100 Gms	EUR 9.41
Description: Part D