Rapid viral antigen test kit for the detection of SARS-CoV-2 in human oropharyngeal, nasal and nasopharyngeal swabs. The test is a lateral flow test with a colloidal gold indicator, giving results in ten minutes.
This rapid SARS-CoV-2 (colloidal gold) antigen test kit uses the sandwich immunocapture method and colloidal gold immunochromatography to qualitatively determine the presence of SARS-CoV-2 antigens. During the test, the sample is dropped into the sample well and the SARS-CoV-2 antigens in the sample are bound by colloidal gold-labeled anti-SARS-CoV-2 monoclonal antibodies.
These then migrate together to the test area as the capillary effect draws the liquid onto the nitrocellulose test strip. A row of SARS-CoV-2 monoclonal antibodies are coated onto the strip in the test area (T line), and these also bind to antigens. The Antibody-Antigen-Antibody Colloidal Gold Sandwich results in a visible line on the T line if SARS-CoV-2 antigens are present in the sample.
The quality control area (line C) is coated with goat anti-mouse antibodies that bind to colloidal gold-labeled antibodies, resulting in a visible Cline. If line C does not show any color, it indicates that the result is invalid and that this patient needs to be retested.
